1. Large-scale generation of human allodepleted anti-3rd party lymphocytes
- Author
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De Ioanni, Maria, Di Ianni, Mauro, Bonifacio, Elisabetta, Moretti, Lorenzo, Cecchini, Debora, Bazzucchi, Federico, Terenzi, Adelmo, Aloisi, Teresa, Falzetti, Franca, Aversa, Franco, Reisner, Yair, Martelli, Massimo F., and Tabilio, Antonio
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LEUCOCYTES , *LYMPHOCYTES , *GRAFT versus host disease , *GRAFT versus host reaction - Abstract
Abstract: Although adoptive transfer of donor lymphocytes protects from infections and relapse after allogeneic hematopoietic stem cell transplantation in both mice and in men, it is associated with a high risk of graft versus host disease (GvHD) which rises with HLA mismatching and the number of T lymphocytes that are infused. Elimination/reduction of alloreactive donor T lymphocytes is an appealing approach and several strategies have been proposed. Here we describe generation of anti-3rd party T lymphocytes under conditions of IL-2 deprivation and their effects in a pre-clinical murine model. Our results clearly indicated that anti-3rd party T lymphocytes generated on a large scale by means of IL-2 deprivation maintain a broad T cell repertoire, do not proliferate in a mixed lymphocyte reaction and do not cause GvHD in NOD–SCID mice. These anti-3rd party lymphocytes contain a large adaptive T regulatory cell subset which might contribute to in vitro and in vivo immune modulation. [Copyright &y& Elsevier]
- Published
- 2008
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