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Fungi are a common problem in the photographic collection, so the aim of this study focused on isolating and molecular identification of fungi from old albumen prints dating to an archive of Dr. Francis and belonging to the Al-Hagar Family and dating back to 1880–1890. The isolated fungi were identified according to their morphological traits and PCR sequencing. The ability of these isolates to cause deterioration was evaluated on model samples (2 × 2 cm) of albumen silver prints. The effect of these fungi on the morphology and structure of the tested samples were examined by SEM, ATR-FTIR, and chromatic alternations. Four fungal species Aspergillus sydowii , A. flavus , Talaromyces atroroseus , and Penicillium chrysogenum were identified. All isolates were able to grow on the surface of the model Albumen silver print and were capable of causing damage to the binder and able to extend their growth to the paper fibers. A. sydowii , A. flavus , and P. chrysogenum caused hydrolysis and oxidation to the albumen prints, while no significant chemical damage to the albumen was detected for the photographic sample infected with T. atroroseus. All the inoculated samples were significantly affected in terms of color change and the high-light areas have become darker. ATR-FTIR spectra showed the degradation of the protein content in Albumen silver prints inoculated with A. sydowii , A. flavus , and P. chrysogenum. [Display omitted] • Isolation and molecular identification of fungi from old albumen prints. • A. sydowii , A. flavus , T. atroroseus , and P. chrysogenum were identified. • A. sydowii , A. flavus , and P. chrysogenum caused hydrolysis and oxidation of albumen prints. • Model albumen prints affected significantly in color changes. • A. sydowii , A. flavus , and P. chrysogenum caused degradation of the protein content. [ABSTRACT FROM AUTHOR]