1. Nitric oxide and bcl-2 mediated the apoptosis induced by nickel(II) in human T hybridoma cells
- Author
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Guan, Fuqin, Zhang, Dongmei, Wang, Xinchang, and Chen, Junhui
- Subjects
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NITRIC oxide , *NITROGEN compounds , *APOPTOSIS , *HYBRIDOMAS - Abstract
Abstract: Although effects of nickel(II) on the immune system have long been recognized, little is known about the effects of nickel(II) on the induction of apoptosis and related signaling events in T cells. In the present study, we investigated the roles and signaling pathways of nickel(II) in the induction of apoptosis in a human T cell line jurkat. The results showed that the cytotoxic effects of Ni involved significant morphological changes and chromosomal condensation (Hoechst 33258 staining). Analyses of hypodiploid cells and FITC–Annexin V and PI double staining showed significant increase of apoptosis in jurkat cells 6, 12 and 24 h after nickel(II) treatment. Flow cytometry analysis also revealed that the loss of mitochondrial membrane potential (MMP) occurred concomitantly with the onset of NiCl2-induced apoptosis. Induction of apoptotic cell death by nickel was mediated by reduction of bcl-2 expression. Furthermore, nickel stimulated the generation of nitric oxide (NO). These results suggest that nickel(II) chloride induces jurkat cells apoptosis via nitric oxide generation, mitochondrial depolarization and bcl-2 suppression. [Copyright &y& Elsevier]
- Published
- 2007
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