1. Expression of bioactive human M-CSF soluble receptor in transgenic tobacco plants
- Author
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Zheng, Guo-Guang, Yang, Ying-Hua, Rao, Qing, Lin, Yong-Min, Zhang, Bin, and Wu, Ke-Fu
- Subjects
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TOBACCO , *TRANSGENIC organisms , *AGROBACTERIUM , *ENZYME-linked immunosorbent assay - Abstract
Abstract: The cDNA encoding N-terminal three immunoglobin-like domains of human M-CSFR was linked to His-tag and endoplasmic reticulum retention sequence (KDEL) before being inserted into the genome of tobacco plant, Nicotiana tabacum cv. NC-89, by Agrobacterium tumefaciens-mediated transformation. The insertion and expression of target gene were confirmed by PCR, ELISA, and Western blot. The recombinant M-CSFsR reached a maximum expression level of 1.92% of total soluble protein in transgenic tobacco plant leaf tissues. The recombinant M-CSFsR could be purified through a one-step IMAC process and its bioactivity was confirmed by the inhibition of colony formation of J6-1 cells. The results suggested that we successfully expressed a high level of bioactive human M-CSFsR in tobacco plants. [Copyright &y& Elsevier]
- Published
- 2006
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