1. Production of hepatitis B virus-infected human B-cell hybridomas: transmission of the viral genome to normal lymphocytes in cocultures.
- Author
-
Colucci G, Lyons P, Beazer Y, and Waksal SD
- Subjects
- Cell Line, DNA, Viral biosynthesis, Hepatitis B Antigens biosynthesis, Hepatitis B virus physiology, Humans, Hybridomas immunology, Immunoglobulin G biosynthesis, Immunoglobulin M biosynthesis, Plasmids, RNA, Viral biosynthesis, Virus Cultivation, Virus Replication, B-Lymphocytes microbiology, Hepatitis B virus isolation & purification, Hybridomas microbiology, T-Lymphocytes microbiology
- Abstract
The presence of hepatitis B virus (HBV) genome and transcripts in mononuclear cells from a patient with acute type B hepatitis offered the possibility of developing a cell line which could serve as a model for HBV replication in lymphocytes. A human B-cell hybridoma, KDG92, was then produced which carries HBV DNA in an episomal state and expresses the major virus transcripts as well as its surface (HBsAg), core (HBcAG), and e (HBeAg) antigens. KDG92 releases in the supernatant surface antigen particles but not core or Dane particles. However, in cocultures this hybridoma is able to transmit episomal HBV DNA to normal lymphocytes, both T and B cells. This in vitro system can therefore provide important indications as to the virus life cycle in lymphocytes and the mechanisms of virus propagation from cell to cell.
- Published
- 1988
- Full Text
- View/download PDF