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A library of composite polymer networks (CPNs) were formed by combining Pluronic F127, as the primary gelator, with a range of di-acrylate functionalised PEG polymers, which tune the rheological properties and provide UV crosslinkability. A coarse-grained sol-gel room temperature phase diagram was constructed for the CPN library, which identifies PEG-dependent disruption of micelles as leading to liquefication. Small angle X-ray scattering and rheological measurements provide detailed insight into; (i) micelle-micelle ordering; (ii) micelle-micelle disruption, and; (iii) acrylate-micelle disruption; with contributions that depend on composition, including weak PEG chain length and end group effects. The influence of composition on 3D extrusion printability through modulation of the cohesive/hydrophobic interactions was assessed. It was found that only micelle content provides consistent changes in printing fidelity, controlled largely by printing conditions (pressure and feed rate). Finally, the hydrogels were shown to be UV photo-crosslinkable, which further improves fidelity and structural integrity, and usefully reduces the mesh size. Our results provide a guide for design of 3D-printable CPN inks for future biomedical applications., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Dynamic microscopy of living cells and organisms alone does not reveal the high level of complexity of cellular and subcellular organization. All observable processes rely on the activity of biochemical and biophysical processes and many occur at a physiological equilibrium. Experimentally, it is not trivial to apply a perturbation that targets a specific process without perturbing the overall equilibrium of a cell. Drugs and more recently RNAi certainly have general and undesired effects on cell physiology and metabolism. In particular, they affect the entire cell. Pulsed lasers allow to severe biological tissues with a precision in the range of hundreds of nanometers and to achieve ablation on the level of a single cell or a subcellular compartment. In this chapter, we present an efficient implementation of a picosecond UV-A pulsed laser-based nanosurgery system and review the different mechanisms of ablation that can be achieved at different levels of cellular organization. We discuss the performance of the ablation process in terms of the energy deposited onto the sample and compare our implementation to others recently employed for cellular and subcellular surgery. Above the energy threshold of ionization, we demonstrate how to achieve single-cell ablation through the induction of mechanical perturbation and cavitation in living organisms. Below this threshold, we induce cytoskeleton severing inside live cells. By combining nanosurgery with fast live-imaging fluorescence microscopy, we show how the apparent equilibrium of the cytoskeleton can be perturbed regionally inside a cell.