1. The magnitude of cytokine production by stimulated CD56 + cells is associated with early stages of systemic sclerosis.
- Author
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Cossu M, van Bon L, Nierkens S, Bellocchi C, Santaniello A, Dolstra H, Beretta L, and Radstake TR
- Subjects
- Adult, Aged, CD56 Antigen immunology, Female, Humans, Leukocytes, Mononuclear immunology, Male, Middle Aged, Toll-Like Receptor 1 immunology, Toll-Like Receptor 2 immunology, Cytokines immunology, Scleroderma, Systemic immunology
- Abstract
Immune activation is a hallmark of systemic sclerosis (SSc). However, the immunological alterations that occur in preclinical and non-fibrotic SSc and that differentiate these subjects from those with primary Raynaud's phenomenon (PRP) or healthy controls (HC) are poorly defined. We isolated CD56
+ (NK/NKT-like) cells from HC, patients with PRP, early SSc (EaSSc) and definite SSc without skin or lung fibrosis. Cytokine production upon different activating stimuli was measured via a multiplex immuno assay. Clearly discriminative patterns among the different stages of SSc were most markedly observed after TLR1/2 stimulation, with increased IL-6, TNF-α and MIP-1α/CCL3 production in definite SSc patients as compared to HC and/or PRP. Initial alterations were observed in EaSSc patients with an intermediate secretion pattern between HC/PRP and definite SSc. CD56+ cells from patients at different stages of SSc differentially respond to TLR stimulation, highlighting the relevance of natural immunity in the developmental and pre-fibrotic SSc., (Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2016
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