1. Comprehensive mapping of immunodominant and conserved serotype- and group-specific B-cell epitopes of nonstructural protein 1 from dengue virus type 1.
- Author
-
Chen Y, Pan Y, Guo Y, Qiu L, Ding X, and Che X
- Subjects
- Animals, Antibodies, Monoclonal immunology, Blotting, Western, Cell Line, Chromosome Mapping, Conserved Sequence genetics, Cross Reactions genetics, Cross Reactions immunology, Dengue Virus classification, Dengue Virus immunology, Epitopes, B-Lymphocyte immunology, Immunodominant Epitopes immunology, Mice, Sequence Alignment, Serotyping, Viral Proteins genetics, Viral Proteins immunology, Dengue Virus genetics, Epitopes, B-Lymphocyte genetics, Immunodominant Epitopes genetics
- Abstract
The dengue virus (DENV) nonstructural protein 1 (NS1) is an immunogenic protein that holds potential for the development of vaccines and diagnostic reagents; however, the epitopes of NS1 have not been comprehensively mapped. We mapped B-cell linear epitopes on NS1 using 149 monoclonal antibodies with DENV serotype specificity and cross-reactivity as well as antisera from 27 mice immunized with the four DENV serotypes. Epitope recognition analysis was performed using a set of 15-mer sequential overlapping peptides that spanned the entire NS1 protein from DENV-1. This strategy identified three regions of NS1 that are DENV-1 serotype-specific epitopes, namely amino acid residues 1-15, 71-85, and 338-352. We also identified five group-specific B-cell epitopes that were highly conserved among isolates of the four DENV serotypes. These novel immunodominant serotype- and group-specific B-cell epitopes of DENV NS1 may aid the development of new dengue vaccines and diagnostic assays., (Copyright 2009 Elsevier Inc. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF