1. Transcription, translation, and cellular localization of PDV-E66: a structural protein of the PDV envelope of Autographa californica nuclear polyhedrosis virus.
- Author
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Hong T, Braunagel SC, and Summers MD
- Subjects
- Amino Acid Sequence, Base Sequence, Genes, Viral genetics, Molecular Sequence Data, Molecular Weight, Nuclear Envelope microbiology, Nuclear Envelope ultrastructure, Nucleopolyhedroviruses genetics, Nucleopolyhedroviruses physiology, Nucleopolyhedroviruses ultrastructure, Open Reading Frames, RNA, Messenger analysis, RNA, Viral analysis, Viral Envelope Proteins chemistry, Viral Envelope Proteins isolation & purification, Viral Structural Proteins chemistry, Viral Structural Proteins isolation & purification, Virion ultrastructure, Nucleopolyhedroviruses chemistry, Transcription, Genetic, Viral Envelope Proteins genetics, Viral Structural Proteins genetics, Virion chemistry
- Abstract
A late gene encoding a 66-kDa structural protein of Autographa californica nuclear polyhedrosis virus was mapped and sequenced (26.9-29.7 MU). Transcription initiates from two conserved TAAG motifs (-15 and -37) with transcripts detected from 12 to 72 hr pi. The protein is detected in infected Spodoptera frugiperda (Sf9) cells from 24 to 72 hr pi. Western-blot and immunoelectron microscopic data identify this protein as specific for the polyhedra-derived virus (PDV) envelope. This protein has been named PDV-E66 to identify its viral origin, envelope location, and apparent molecular weight. In addition to being a structural protein of the PDV envelope, PDV-E66 is enriched in foci of microvesicles in the nuclei of infected Sf9 cells.
- Published
- 1994
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