1. Microbial expression of Exendin-4 analog and its efficacy in mice model.
- Author
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Kodaganti BP, Mukunda P, Dakshinamurthy P, Manjunath Y, Shenoy BR, Kamanagowda V, Natarajan B, Maliwalave A, Unnikrishnan D, Murugesan S, Halan V, Ghosh M, and Maity S
- Subjects
- Animals, Drug Evaluation, Preclinical, Exenatide, Male, Mice, Recombinant Proteins biosynthesis, Recombinant Proteins genetics, Recombinant Proteins isolation & purification, Recombinant Proteins pharmacology, Escherichia coli, Gene Expression, Peptides genetics, Peptides isolation & purification, Peptides pharmacology, Venoms biosynthesis, Venoms genetics, Venoms isolation & purification, Venoms pharmacology
- Abstract
Exendin-4 is a GLP 1 agonist incretin-mimetic peptide hormone comprising 39 amino acids. Exenatide (Byetta
® ) is a chemically synthesized version of Exendin-4 with an additional C-terminal amidation. Exenatide acts as a GLP-1 receptor agonist. This paper illustrates the method adopted for cloning, fermentation and purification of recombinant Exendin-4 analog expressed in Escherichia coli. The biologically expressed analog was extensively characterized using different orthogonal methods to confirm their biological activity and physicochemical properties. It was observed that the expressed analog showed comparable functional properties as that of Byetta® irrespective of their modes of development. Further, in vivo efficacy of the recombinant Exendin-4 analog was studied in Oral Glucose Tolerance Test (OGTT) in mice models. Byetta® and Exendin-4 analog treated groups showed comparable glucose lowering activity in the OGTT model., (Copyright © 2017 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.)- Published
- 2017
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