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The present work was designed to assess the potential hemato-biochemical protective action, immunemodulatory and antioxidant conclusions of varied concentration of white mulberry Morus alba leaves (MAL) extract supplementation on Nile tilapia (Oreochromis .niloticus). A total two hundred and forty of O. niloticus were haphazardly sorted into four groups. The control (CT) group was fed on basal diet. A group MAL 1 , MAL 3 and MAL 5 was fed on 1, 3 and 5 g/kg MAL respectively for thirty days. On day thirty one, half of replicates in each group were challenged by 0.5 ml × 10 8 Aeromonas hydrophila where, the residual replicates were kept without challenge. A. hydrophila challenged tilapias revealed anemia that alleviated by supplementation with 5 g/kg MAL also, recovers the shift of leucogram prompted by the challenge. Elevation of alkaline phosphatase, aminotransferases, lactate dehydrogenase and malondialdehyde (ALP, ALT, AST, LDH and MDA) in CT, MAL 1 and MAL 3 in the challenged replicates respectively where within normal at MAL 5 . Supplementation with MAL 5 showed more potent antioxidant and immune reaction than MAL 1 and MAL 3 . There were a rapid increase of immunoglobulin M, lysozymes, nitric oxide, catalase and superoxide dismutase and their allied genes expression (IgM, CAT and SOD) in MAL groups with contrast in CT challenged groups. Where in challenged groups, there was suppression in genes expression of interleukins (8 and 1 beta) and interferon ɤ (IL8. IL-1β and INFɤ). Tilapias challenged by A. hydrophila unveiled plentiful surge in the percentage of mortality in CT challenged fish (80%), followed by the groups supplemented with MAL 1 and MAL 3 were (73.33%) where MAL 5 was 20%. The mortalities have been halted from the 6 th , 13 th , 14 th and 15 th days in, MAL 5 , MAL 3 , MAL 1 , and CT correspondingly. These previous results could be fulfilled that using of MAL 5 g/kg protect tilapias from hemato-biochemical alterations and enhance its immune feedback, antioxidant defense and resistance against A. hydrophila., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

The toxic effect of deltamethrin (DM) was documented in aquaculture. There is no obtainable data on the effect of Chlorella vulgaris against DM toxicity. The current study focused on the effect of dietary supplementation with C. vulgaris (CV) on growth performance, innate immune response, antioxidant activities, and transcriptomics disorders induced by sub-lethal dose of DM in Oreochromis niloticus. A total number of 216 O. niloticus divided into four groups with tri-replicates. The 1 st control group (CT) fed a basal diet, the second group fed diet enriched with 5% CV. The third group was exposed to DM (15 μg/L), where the last group fed CV and simultaneously exposed to DM as previous-mentioned. The procedure of CV feeding and DM exposure were continued for two months. Exposures to DM revealed in stunting of the growth parameters and lessening of survival ratio of tilapias with a significant decline of the erythrogram (macrocytic hypochromic anemic picture), and leucocytes immune cells and related parameters (immunoglobulin M, lysozyme) and sever shifting in the antioxidant indicators. Sever raise was monitored in hepatic and kidney markers. Also, genes expression related to immune and antioxidant parameters were severely impacted. Where tilapias received CV showed a significant increase in the growth and immune parameters besides to an improvement of hematological, antioxidant values and their related genes expressions. The fourth group that received CV simultaneous with DM exposure showed a soothing of the previous indicators and markers toward the values of tilapias fed on basal diet (CT). In turn, CV supplementation may be presented a protective effect alongside DM toxicity in O. niloticus appeared through soothing of the immune, antioxidant and related genes expressions in addition to its hepato-renal protective effects. Therefore, the current study recommended that an incorporating of 5% CV for tilapias diet could improve their growth performance, immunity, antioxidant and transcriptomics disorders induced by deltamethrin., Competing Interests: Declaration of competing interest None., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

As recently applicable, there are few studies on the impact of using nano-selenium (nano-Se) on varied fish species. Where nothing reachable focused on its impact on tilapias so, the present analysis evaluated the efficacy of using nano-Se in tilapias on immune response, antioxidant defense compared by conventional Se form. 480 O. niloticus fingerlings were haphazardly grouped firstly into three groups with four replicates of each. The control one (CT) was fed on a basal diet. The second and third one supplemented with 0.7 mg/kg -1 Se and nano-Se respectively for ten weeks. At the start day of the ninth week, two replicates from each group were injected by Streptococcus iniae where, the remaining replicates stand without challenge. Enhancement of growth performance measurements were noted in nano-Se compared to Se or CT groups. Existed anemia in S. iniae tilapias became alleviated by using nano-Se that also, improves the alteration of leucogram induced by challenge. Elevation of aminotransferases, alkaline phosphatase, lactate dehydrogenase (ALT, AST, ALP and LDH) and creatinine in Se and CT challenged replicates that seemed nearly normal by using nano-Se. Usage of nano-Se showed more powerful antioxidant activities than Se. There were an expansion of immunoglobulin M, lysozymes, glutathione peroxidase, nitric oxide, superoxide dismutase and catalase (IgM, LYZ, GPx, NO, SOD, CAT) and their related gene expression in nano-Se with contrast in Se or CT challenged groups. Nile tilapias challenged by S. iniae disclosed substantial expansion in the percentage of mortality in CT challenged fish (93.33%), followed by the group supplemented with Se (73.33%), whereas the lowermost one at fish supplemented by nano-Se (26.66%). The mortalities have been stopped from the 5 th , 12 th and 14 th days in, nano-Se, Se and CT respectively. It can be concluded that using of Se 0.7 mg/kg -1 induce immunosuppressive, antioxidant, liver and kidneys negative impact on tilapias where the same dose from nano-Se was more potent immunomodulating and antioxidant. Also it is attend in counteracting the serious impact induced by S. iniae challenge., (Copyright © 2019 Elsevier Ltd. All rights reserved.)