1. Administration of recombinant Reishi immunomodulatory protein (rLZ-8) diet enhances innate immune responses and elicits protection against nervous necrosis virus in grouper Epinephelus coioides.
- Author
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Kuan YC, Sheu F, Lee GC, Tsai MW, Hung CL, and Nan FH
- Subjects
- Animals, Cells, Cultured, Cytokines genetics, Dietary Supplements, Fungal Proteins, Gene Expression Regulation drug effects, Leukocytes drug effects, Nodaviridae, Phagocytosis drug effects, RNA Virus Infections immunology, Recombinant Proteins pharmacology, Reishi chemistry, Respiratory Burst drug effects, Diet veterinary, Fish Diseases immunology, Immunity, Innate drug effects, Immunologic Factors pharmacology, RNA Virus Infections veterinary, Reishi immunology
- Abstract
Nervous necrosis virus (NNV) infection during larvae and juvenile stage in grouper (Epinephelus coioides) has caused severe economic losses in the aquaculture industry in Asia. The aims of this study were to evaluate the influence of recombinant Reishi protein, rLZ-8, on the innate immune responses and the viral resisting ability in fish. Groupers were fed with rLZ-8 supplemented diet (1.25-37.5 mg (rLZ-8)/kg(diet)), and the cytokine gene expression, innate immune responses, and survival rate after NNV challenge were examined. The fish fed with rLZ-8 diet showed 6- to 11-fold upregulated TNF-α and IL-1β gene expression, along with significant increased respiratory burst and phagocytic activity. Moreover, feeding the fish with 37.5 mg/kg rLZ-8 diet elicited significant improvement in post viral challenge survival rate (85.7%). These discoveries indicated that rLZ-8 could be utilized as an ant-pathogen immunostimulant, and provided a new candidate to fight against NNV infection in fish., (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Published
- 2012
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