1. Selection and structural analysis of the NY25 peptide - A vaccine candidate from hemagglutinin of swine-origin Influenza H1N1.
- Author
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Khrustalev VV, Khrustaleva TA, and Kordyukova LV
- Subjects
- Antibodies, Viral blood, Circular Dichroism, Computational Biology, Conserved Sequence, Hemagglutinin Glycoproteins, Influenza Virus genetics, Humans, Influenza A Virus, H1N1 Subtype genetics, Influenza Vaccines chemistry, Influenza Vaccines isolation & purification, Models, Molecular, Oligopeptides chemistry, Protein Conformation, Vaccines, Subunit chemistry, Vaccines, Subunit immunology, Vaccines, Subunit isolation & purification, Hemagglutinin Glycoproteins, Influenza Virus immunology, Influenza A Virus, H1N1 Subtype immunology, Influenza Vaccines immunology, Influenza, Human prevention & control, Oligopeptides immunology
- Abstract
The aim of this study was to construct a vaccine peptide candidate against pandemic Influenza H1N1 hemagglutinin and to test its structure. With the help of bioinformatic algorithms we showed that the sequence encoding the second polypeptide of pandemic Influenza H1N1 hemagglutinin (HA2) is protected from nonsynonymous mutations better than the sequence encoding its first polypeptide (HA1). With the help of secondary and ternary structure predicting algorithms we found the fragment of HA2 with the most reproducible secondary structure and synthesized the NY25 peptide corresponding to the residues Asn117 - Tyr141 of HA2. According to the circular dichroism spectra analysis, the peptide has short helix and beta hairpin. According to the analysis of differential fluorescence quenching results, two tyrosine residues are situated on a long distance from each other. These facts taken together with the positive results of affine chromatography with the serum of a person immunized by full-length hemagglutinin confirm that the structure of the fragment of viral full-length protein has been reproduced in the synthetic NY25 peptide. Amino acid sequence of the NY25 peptide (NLYEKVRSQLKNNAKEIGNGCFEFY) is relatively conserved in 18 subtypes of Influenza A virus hemagglutinin., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Published
- 2018
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