1. Hematopoietic differentiation of embryonic stem cells.
- Author
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Orlovskaya I, Schraufstatter I, Loring J, and Khaldoyanidi S
- Subjects
- Animals, Cell Culture Techniques methods, Cell Differentiation drug effects, Cell Line metabolism, Cell Lineage genetics, Cell Separation methods, Culture Media pharmacology, Culture Media standards, Diffusion Chambers, Culture, Hematopoiesis, Hematopoietic System physiology, Humans, Mice, Stromal Cells cytology, Stromal Cells drug effects, Cell Differentiation physiology, Embryonic Stem Cells drug effects, Embryonic Stem Cells physiology, Hematopoietic Stem Cells cytology, Hematopoietic Stem Cells physiology
- Abstract
This review is focused on methods that are used to derive hematopoietic cells from embryonic stem cells (ESCs). One of the strategies that have been recently used to achieve this goal is an approach of mimicking the hematopoietic niche in vitro by using hematopoiesis-supportive feeder cells, cocktails of soluble hematopoietic growth factors and a variety of matrices. While there is clear evidence that it is possible to derive hematopoietic stem cells (HSCs) and subsequently committed hematopoietic progenitors and mature cells from ESCs, there remains the need to address multiple issues including the efficiency of HSCs derivation in vitro and their proper functionality.
- Published
- 2008
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