1. A PCR-RFLP method for the simultaneous differentiation of three Entamoeba species.
- Author
-
Fontecha GA, García K, Rueda MM, Sosa-Ochoa W, Sánchez AL, and Leiva B
- Subjects
- Deoxyribonucleases, Type II Site-Specific, Diagnosis, Differential, Entamoeba genetics, Entamoeba isolation & purification, Entamoeba histolytica classification, Entamoeba histolytica genetics, Entamoeba histolytica isolation & purification, Entamoebiasis parasitology, Humans, Polymerase Chain Reaction standards, Entamoeba classification, Entamoebiasis diagnosis, Polymerase Chain Reaction methods, Polymorphism, Restriction Fragment Length, Restriction Mapping
- Abstract
Amoebiasis caused by Entamoeba histolytica continues to be one of the most common parasitic diseases in the developing world. Despite its relevance, due to the lack of accurate diagnostic methods, the true clinical and public health importance of this parasite remains uncertain. The aim of this study was to develop a new diagnostic tool to differentiate E.histolytica from the morphologically undistinguishable E.dispar and E.moshkovskii. We developed a specific, fast and simple PCR-RFLP method that was able to accurately differentiate experimentally-obtained restriction patterns from the three Entamoeba species. This new method could prove useful for clinical and epidemiological purposes., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF