1. Vernonia DGATs can complement the disrupted oil and protein metabolism in epoxygenase-expressing soybean seeds.
- Author
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Li R, Yu K, Wu Y, Tateno M, Hatanaka T, and Hildebrand DF
- Subjects
- Vernonia enzymology, Diacylglycerol O-Acyltransferase biosynthesis, Diacylglycerol O-Acyltransferase genetics, Oxidoreductases biosynthesis, Oxidoreductases genetics, Plant Oils metabolism, Plant Proteins genetics, Plant Proteins metabolism, Plants, Genetically Modified enzymology, Plants, Genetically Modified genetics, Seeds enzymology, Seeds genetics, Glycine max enzymology, Glycine max metabolism, Vernonia genetics
- Abstract
Plant oils can be useful chemical feedstocks such as a source of epoxy fatty acids. High seed-specific expression of a Stokesia laevis epoxygenase (SlEPX) in soybeans only results in 3-7% epoxide levels. SlEPX-transgenic soybean seeds also exhibited other phenotypic alterations, such as altered seed fatty acid profiles, reduced oil accumulation, and variable protein levels. SlEPX-transgenic seeds showed a 2-5% reduction in total oil content and protein levels of 30.9-51.4%. To address these pleiotrophic effects of SlEPX expression on other traits, transgenic soybeans were developed to co-express SlEPX and DGAT (diacylglycerol acyltransferase) genes (VgDGAT1 & 2) isolated from Vernonia galamensis, a high accumulator of epoxy fatty acids. These side effects of SlEPX expression were largely overcome in the DGAT co-expressing soybeans. Total oil and protein contents were restored to the levels in non-transgenic soybeans, indicating that both VgDGAT1 and VgDGAT2 could complement the disrupted phenotypes caused by over-expression of an epoxygenase in soybean seeds., (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Published
- 2012
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