Search

Your search keyword '"Daffis S"' showing total 2 results
Start Over Author "Daffis S" Publisher academic press
2 results on '"Daffis S"'

1. Genetic identification of Kodoko virus, a novel arenavirus of the African pigmy mouse (Mus Nannomys minutoides) in West Africa.

Author

Lecompte E, ter Meulen J, Emonet S, Daffis S, and Charrel RN

Subjects
Africa, Western, Animals, Arenaviridae Infections virology, Arenaviruses, Old World classification, Arenaviruses, Old World isolation & purification, Base Sequence, DNA Primers genetics, Evolution, Molecular, Humans, Lymphocytic choriomeningitis virus classification, Lymphocytic choriomeningitis virus genetics, Molecular Sequence Data, Phylogeny, RNA, Viral genetics, Species Specificity, Zoonoses virology, Arenaviruses, Old World genetics, Mice virology
Abstract

Genetic evidence of a novel arenavirus species related to but distinct from lymphocytic choriomeningitis virus (LCMV) was obtained from a rodent belonging to an endemic African subgenus of Mus (Nannomys). The phylogenetic position among Old World arenaviruses of this new arenavirus, named Kodoko virus, was reconstructed based on L and NP genes sequences. The finding of an Old World arenavirus related to LCMV outside the known geographical range of LCMV in a novel rodent species reveals new insights about the evolutionary history of arenaviruses.

Published
2007
Full Text
View/download PDF

2. Antibody responses against wild-type yellow fever virus and the 17D vaccine strain: characterization with human monoclonal antibody fragments and neutralization escape variants.

Author

Daffis S, Kontermann RE, Korimbocus J, Zeller H, Klenk HD, and Ter Meulen J

Subjects
Amino Acid Sequence, Antibodies, Monoclonal analysis, Antibodies, Viral analysis, Antibodies, Viral chemistry, Gene Products, env chemistry, Gene Products, env genetics, Gene Products, env immunology, Humans, Immunoglobulin Heavy Chains chemistry, Immunoglobulin Light Chains chemistry, Models, Molecular, Molecular Sequence Data, Neutralization Tests, Protein Conformation, Viral Matrix Proteins chemistry, Viral Matrix Proteins genetics, Viral Matrix Proteins immunology, Antibodies, Monoclonal genetics, Antibodies, Viral genetics, Antibody Formation, Genetic Variation, Peptide Fragments immunology, Yellow Fever Vaccine immunology, Yellow fever virus genetics, Yellow fever virus immunology
Abstract

Human monoclonal antibody fragments neutralizing wild-type and vaccine strains of yellow fever (YF) virus (genotypes West Africa I + II, East/Central Africa, 17D-204-WHO) were generated by repertoire cloning from YF patients. Analysis of virus escape variants identified amino acid (aa) 71 in domain II of the envelope glycoprotein (E) as the most critical residue for neutralization, with aa 153-155 in domain I contributing to the epitope. These data confirm the previous mapping of YFV neutralizing epitopes using mouse monoclonal antibodies but suggest that a conformational epitope could be formed by amino acids from domains I and II opposing each other in the dimeric form of the E protein. While the sera of the YF patients showed up to 10-fold reduced neutralizing activity against the 17D escape variants, sera from 17D vaccinees retained their neutralizing titers. Mutations in this major neutralizing epitope of YFV thus do not seem to carry the risk of immune escape in persons immunized with the YFV-17D vaccine.

Published
2005
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results