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1. Selection of Pseudomonas aeruginosa reference genes for RT-qPCR analysis from sputum of cystic fibrosis patients.

Author

Costaglioli P, Barthe C, Fayon M, Christoflour N, Bui S, Derlich L, Domblides P, Crouzet M, Vilain S, and Garbay B

Subjects
Gene Expression Profiling methods, Humans, Real-Time Polymerase Chain Reaction, Cystic Fibrosis microbiology, Genes, Bacterial, Pseudomonas aeruginosa genetics, Sputum microbiology
Abstract

The prerequisite to monitor gene expression is the selection of reference genes for normalization of RT-qPCR results. Using 13 sputum samples collected from 9 CF patients, we demonstrated that PA2875 and PA3340 are better reference genes than the previously used clpX and oprL genes., (Copyright © 2013 Elsevier Ltd. All rights reserved.)

Published
2014
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