1. Dextran sulfate sodium upregulates MAPK signaling for the uptake and subsequent intracellular survival of Brucella abortus in murine macrophages.
- Author
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Reyes AW, Arayan LT, Simborio HL, Hop HT, Min W, Lee HJ, Kim DH, Chang HH, and Kim S
- Subjects
- Animals, Brucella abortus physiology, Brucellosis genetics, Brucellosis metabolism, Host-Pathogen Interactions, Humans, MAP Kinase Signaling System drug effects, Macrophages drug effects, Macrophages metabolism, Mice, Microbial Viability drug effects, RAW 264.7 Cells, Up-Regulation drug effects, Bacterial Adhesion drug effects, Brucella abortus drug effects, Brucella abortus growth & development, Brucellosis microbiology, Dextran Sulfate pharmacology, Macrophages microbiology
- Abstract
Brucellosis is one of the major zoonoses worldwide that inflicts important health problems in animal and human. Here, we demonstrated that dextran sulfate sodium (DSS) significantly increased adhesion of Brucella (B.) abortus in murine macrophages compared to untreated cells. Even without infection, Brucella uptake into macrophages increased and F-actin reorganization was induced compared with untreated cells. Furthermore, DSS increased the phosphorylation of MAPKs (ERK1/2 and p38α) in Brucella-infected, DSS-treated cells compared with the control cells. Lastly, DSS markedly increased the intracellular survival of Brucella abortus in macrophages by up to 48 h. These results suggest that DSS enhanced the adhesion and phagocytosis of B. abortus into murine macrophages by stimulating the MAPK signaling proteins phospho-ERK1/2 and p38α and that DSS increased the intracellular survival of B. abortus by inhibiting colocalization of Brucella-containing vacuoles (BCVs) with the late endosome marker LAMP-1. This study emphasizes the enhancement of the phagocytic and intracellular modulatory effects of DSS, which may suppress the innate immune system and contribute to prolonged Brucella survival and chronic infection., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2016
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