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1. Ectoine biosynthesis genes from the deep sea halophilic eubacteria, Bacillus clausii NIOT-DSB04: Its molecular and biochemical characterization.

Author

Anburajan L, Meena B, Sreelatha T, Vinithkumar NV, Kirubagaran R, and Dharani G

Subjects
Bacillus clausii isolation & purification, Chromatography, High Pressure Liquid, Escherichia coli genetics, Escherichia coli metabolism, Gene Expression, Magnetic Resonance Spectroscopy, Multigene Family, Recombinant Proteins genetics, Recombinant Proteins metabolism, Amino Acids, Diamino biosynthesis, Bacillus clausii genetics, Bacillus clausii metabolism, Biosynthetic Pathways genetics, Genes, Bacterial, Seawater microbiology
Abstract

Ectoine, the most prominent osmolyte in nature, is a vital compatible solute present in halophilic bacterium. It protects the cellular biomolecules of the halophilic bacteria and retains their intrinsic function from extreme circumstances. In the current research, ectoine biosynthesis gene cluster (ectABC) in Bacillus clausii NIOT-DSB04 was expressed heterologically in E. coli M15 (pREP4). RP-HPLC resolved several fractions of the purified recombinant product, one of which had been confirmed as ectoine. The recombinant ectoine was further characterized by 1 H and 13 C Nuclear Magnetic Resonance. The purified recombinant ectoine was also authenticated by FT-IR studies with the existence of ester carbonyl and C-H group. In IPTG induced E. coli M15 transgenic cells, the enzymatic activity of the ectA, B and C genes were found to be higher than that of uninduced cells., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Published
2019
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2. Biodiversity and antibacterial potential of cultivable halophilic actinobacteria from the deep sea sediments of active volcanic Barren Island.

Author

Meena B, Anburajan L, Vinithkumar NV, Kirubagaran R, and Dharani G

Subjects
Actinobacteria genetics, DNA, Bacterial genetics, DNA, Ribosomal genetics, Gram-Negative Bacteria pathogenicity, Gram-Positive Bacteria pathogenicity, Islands, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S genetics, Secondary Metabolism, Streptomyces classification, Streptomyces genetics, Actinobacteria classification, Actinobacteria isolation & purification, Actinobacteria metabolism, Anti-Bacterial Agents biosynthesis, Biodiversity, Geologic Sediments microbiology, Seawater microbiology
Abstract

Deep Sea sediment cores were collected from the surrounding of active volcanic Barren Island, Andaman & Nicobar Islands. A total of 123 cultivable marine actinobacteria were isolated and identified based on their biochemical and 16S rDNA sequences. The isolates were categorized under 10 genera, of which Streptomyces sp., Dietzia sp. and Brevibacterium sp. are the dominant genera. Of 123 isolates, 73 isolates exhibited appreciable inhibitory activity against both Gram positive and Gram negative bacteria. High frequencies of positive PCR amplification were obtained for PKS-I (39.13%), PKS-II (56.52%) and NRPS (69.57%). The highest levels of biosynthetic systems were observed for NRPS and PKS-II. Majority of the actinobacterial isolates revealed excellent potential for bioprospecting of novel byproducts with industrial and pharmaceutical importance., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Published
2019
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