1. In vitro regeneration of plantlets from unpollinated ovary culture in sweet orange (Citrus sinensis L. Osbeck)
- Author
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Nighat Seema, Riaz Alam, Mubarak Ali Khan, Nisar Ahmad, Shahid Akbar Khalil, Muhammad Munir Sajid, Hina Fazal, and Roshan Zamir
- Subjects
food and beverages ,Organogenesis ,Orange (colour) ,Biology ,Applied Microbiology and Biotechnology ,In vitro ,Murashige and Skoog medium ,Callus ,Botany ,Shoot ,Genetics ,Jiffy ,Agronomy and Crop Science ,Molecular Biology ,Citrus × sinensis ,Citrus, blood red, ovary culture, callus induction, regeneration, plant growth regulators ,Biotechnology - Abstract
Callogenesis and organogenesis of ovary of sweet orange ( Citrus sinensis ) cv. Blood red was carried on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of N 6 benzyl adenine (BA), 1-napthaleneacetic acid (NAA) and 2,4-D. 1 mg/l BA + 0.5 mg/l NAA on MS medium was the most effective in callus induction and proliferation. Maximum number of shoots (11) was recorded on the medium with 2 mg/l NAA + 3 mg/l BA. The best medium for root induction was MS together with 2.5 mg/l indole-3-acetic acid (IAA) + 2 mg/l indole–3-butyric acid (IBA), where maximum (16) plantlets were rooted. The regenerated plantlets were successfully acclimatized in jiffy pots containing sterilized soil mixture of sand, silt and clay in 1:1:1 ratio to study their response to in vivo conditions. Key words: Citrus, blood red, ovary culture, callus induction, regeneration, plant growth regulators.
- Published
- 2013