Cowpea is popular in Eastern Kenya where it is attractive to farmers because of its high economic value and the belief that it does not require many external inputs. Farmers are however discouraged to grow the crop in this region due to massive attack by a parasitic weed Alectra vogelii (Benth). Yield losses due to A. vogelii have being estimated to range from 50 to 100% in Mbeere, Kitui and Makueni districts. No single method however is available to farmers in these regions in control of the parasitic weed. Combining several control methods, as in the management of Striga spp in Western Kenya should be a sustainable option. Field studies were conducted in 2010 and 2011 at Kenya Agricultural Research Institute (KARI), Kiboko farm to determine the response of 143 cowpea genotypes to Alectra infestation. The aim for the study was to identify resistant genotypes that could be used in breeding programme. Significant differences were observed amongst cowpea genotypes in days to first Alectra emergence, number of Alectra shoots emerged at 6, 8, 10 and 12 week after planting and grain yield. Cowpea genotypes Kir/Nya-005 and Mbe/Mach-022 showed complete resistance to Alectra while Ken-Kunde, M66 and K80 (all commercial varieties) supported the highest number of Alectra shoots. Grain yield loss in the three susceptible varieties was 80, 79 and 50% respectively. On the other hand, Sia/Cia-004, Mbe/Mach-014 and Kib-006 had high grain yields despite the high number of Alectra shoots present. There was a strong correlation (r = -0.57) between grain yield and number of Alectra shoots emerged at 12 weeks after planting. A significant negative (r = -0.37) correlation was also obtained between pod number per plant and number of emerged Alectra shoots at 12 weeks after planting. This negative correlation proves the high accumulation dry matter in the cowpea roots at the expense of the pods thus decreasing grain yield. This information showed that there is sufficient genetic variability in the cowpea genotypes studied, which can be exploited in breeding improved cowpea varieties for resistance to A. vogelii in Kenya. A great progress towards developing improved cowpea variety that meets farmer’s preferences with durable resistance to A. vogelii can be achieved if the genes from the resistant and tolerant local cowpea cultivars identified in this study could be introgressed into the adapted susceptible improved varieties. This will increase the potential impact of adoption of resistant cowpea varieties in the zones. Key words : Cowpea, Alectra vogelii , Resistance/tolerance and grain yield.