1. Chemical composition and antioxidant activity of Lippia alba essential oil obtained by supercritical CO2 and hydrodistillation
- Author
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L. Reyes-Solano, A. P. Breksa, José Basilio Heredia, Miguel Ángel Angulo-Escalante, and J. Benigno Valdez-Torres
- Subjects
Antioxidant ,DPPH ,medicine.medical_treatment ,01 natural sciences ,Applied Microbiology and Biotechnology ,law.invention ,chemistry.chemical_compound ,0404 agricultural biotechnology ,law ,Genetics ,medicine ,Molecular Biology ,Chemical composition ,Essential oil ,Chromatography ,ABTS ,biology ,010405 organic chemistry ,04 agricultural and veterinary sciences ,biology.organism_classification ,040401 food science ,Supercritical fluid ,0104 chemical sciences ,Eucalyptol ,chemistry ,Agronomy and Crop Science ,Lippia alba ,Biotechnology - Abstract
Lippia alba essential oils were obtained by hydrodistillation (HD) and supercritical fluid (SFE) extraction methods. These were analyzed by gas chromatography-mass spectrometry-flame ionization detector (GC-FID-MS). Antioxidant activity was tested by DPPH and ABTS methods, and total soluble phenolics (TSP) were also determined. While in the SFE extract 14 compounds were identified (mostly: myrcenone 3.4%, α-terpineol 1.0% and β-caryophyllene 2.3%), in the HD extract 17 compounds were identified (mostly: eucalyptol 15.6%, myrcenone 9.3% and Z-ocimenone 5.8%). The results showed that L. alba essential oils obtained by SFE was IC50=17.35 mg/mL and by HD was IC50 = 12.45 mg/mL in DPPH assay. L. alba has an excellent eucalyptol content. Key words: Antioxidants, bioactive compounds, supercritical fluid, green technologies.
- Published
- 2017