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This study verified the presence of opportunist pathogenic bacteria in the hands of Surgeons Dentists. Biological materials were collected from the hands of 41 professionals, randomly selected. The professional washed his/her hands for around 30 s in a sterile plastic bag, filled with 250 mL of physiologic serum, in three distinct times: Timing 1 (T1), hands without wearing gloves, before attending to the patient; Timing 2 (T2), wearing gloves, right after the patient treatment, when the glove was contaminated with the patients’ biological material; and Timing 3 (T3), collected after removing the gloves. The material obtained was inoculated in culture mediums such as: Brain Heart Infusion Agar (BHI), Manitol Salt Agar (MSA), Eosin Methylene Blue (EMB) and Bile Esculin Agar (BEA). There were identified Staphylococcus aureus and not aureus strains, besides Gram-negative bacteria, suggesting deficient hygiene of the hands. An antibiogram was made for all the Gram-positive and Gram-negative bacteria found. The result shows a high number of strains resistant to the most common used antibiotics. The microbiological count was higher in the T1 for all the culture mediums, suggesting that the act of washing the hands for 30 s decreases the microbiota resident in the hands. Key words: Antimicrobial agents, hands washing, infection control, odontology, risk due to biological agents.