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The aim of this research was to evaluate the capacity of symbiotic efficiency of native rhizobia from soils of the state of Rio Grande do Sul (RS), Brazil in Phaseolus lunatus L. plants. Soil and nodule samples from predominant legume plants such as Desmodium species were collected in seven locations of the state of RS. For the isolation of the rhizobia from the soil samples, lima bean “olho de cabra preto” variety and “leucena” (Leucena leucocephala) and plants were used as baits. Subsequently, the symbiotic characterization of the isolates was performed by inoculation on lima bean plants in vitro conditions. The selected bacterial isolates were evaluated for biological nitrogen fixation efficiency in a greenhouse experiment, being determined after: shoot dry mass, root dry mass, nodule dry mass, nitrogen (N) accumulated shoot and calculated the N fixation relative efficiency index. Among the 28 rhizobia isolates tested, 11 induced nodule formation in lima bean. The rhizobia Plu03 and Plu14 stimulated a greater increase of plant dry mass, nodule dry mass and nitrogen accumulation in the shoot, reflecting in a higher relative efficiency index. These results suggest that the isolates Plu03 and Plu14 are more efficient in promoting growth of lima bean, which could be recommended for future agronomic efficiency studies. Key words: Rhizobia, lima bean, biological nitrogen fixation.

The soil is the most important carbon reservoir, but agricultural practices involved in tillage systems decrease the soil carbon stock. This study aims to evaluate the effect of no-tillage (NT) on carbon and microbiological attributes of the soil in corn plantations in Manaus-AM, Brazil. Soil was sampled in a secondary forest (SF) and in corn grown under different tillage systems, including conventional tillage (CT), which makes use of plowing and harrowing operations, and no-tillage (NT). Soil variables studied included, carbon content, soil microbial carbon and basal respiration, metabolic and microbial quotients. The data were analyzed by analysis of variance and Tukey's test. The results demonstrated that NT resulted higher carbon, lower basal respiration, and lower metabolic quotient than CT and SF. However, soil microbial carbon was similar in all tillage systems studied. We conclude that NT corn cropping increased soil carbon content more than CT, while it decreased basal respiration and metabolic quotient when compared to NT and SF. Moreover, soil microbial biomass in corn was similar in all tillage systems studied. This research demonstrated the importance of NT to soil carbon conservation and soil management in corn cropping. Key words: Tillage systems, land use, microbial soil biomass, basal respiration.

ability to associate symbiotically with rhizobia. The objective of this study was to isolate, authenticate and select effective rhizobia obtained from native serradella (Ornithopus micranthus) to reduce the need for mineral fertilizer. Nodules, roots of serradella and rhizosphere soil samples were collected in seven municipalities from Rio Grande do Sul and Santa Catarina states. Bacterial colonies were isolated and identified based on morphological characteristics. The authentification and initial selection of rhizobia were conducted in vitro. The most promising strains from the in vitro study were evaluated in a greenhouse experiment for 60 days. Among the 148 bacterial cultures characterized, 113 induced the formation of nodules in serradella while 32 isolate effectively increased fresh mass of plants under in vitro conditions. The strains UFRGS Om57, UFRGS Om59 and UFRGS Om148 formed nodules with greater dry mass and produced high dry mass of plants (shoots and roots) which allowed greater accumulation of N in the shoots. Key words: Plant growth promoting rhizobacteria (PGPR); biological fixation of nitrogen; Ornithopus micrantus; pasture.