1. Isolation, characterization and evaluation of antimicrobial and cytotoxic activity of estragole, obtained from the essential oil of Croton zehntneri (Euphorbiaceae).
- Author
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Andrade TC, De Lima SG, Freitas RM, Rocha MS, Islam T, Da Silva TG, and Militão GC
- Subjects
- Allylbenzene Derivatives, Anisoles isolation & purification, Anti-Infective Agents isolation & purification, Cell Line, Tumor drug effects, Cyclohexanols isolation & purification, Disk Diffusion Antimicrobial Tests, Drug Screening Assays, Antitumor, Eucalyptol, Euphorbiaceae classification, Fungi drug effects, Gas Chromatography-Mass Spectrometry, Gram-Negative Bacteria drug effects, Gram-Positive Bacteria drug effects, Humans, Monoterpenes isolation & purification, Anisoles pharmacology, Anti-Infective Agents pharmacology, Antineoplastic Agents, Phytogenic pharmacology, Croton Oil chemistry, Cyclohexanols pharmacology, Euphorbiaceae chemistry, Monoterpenes pharmacology, Oils, Volatile chemistry
- Abstract
Croton zehntneri (Euphorbiaceae) is a native aromatic plant from Northeast region of Brazil. The monoterpenoid estragole (ESL) has been isolated by classical chromatographic methods from the essential oil (EO) of C. zehnteneri leaves and characterized by GC-FID and GC-MS, its antimicrobial and cytotoxic potentials being assessed. The analysis of the EO enabled the identification of 100% of the integrated constituents, of which yield was about 1.8%. The main components identified were: eucalyptol, estragole (84.7%) and spathulenol. The dosage of 50 μg/disk of ESL presented fairly significant zones of inhibition against Gram-positive bacteria and fungi. The ESL presented toxicity against Artemia salina with LC50 and LC90 of 4,54 and 8,47 μg mL-1. However, in tumor inhibition assays (human cells), there were no rewarding inhibition in any of the human cancer cell lines (MCF-7, HEP-2 and NCI-H292).
- Published
- 2015
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