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Diabetes is a growing problem in modern times. Treating diabetes can be done using various ways, from traditional to modern methods. Herbal medicine is a traditional form of medication. Herbs can be used to treat diabetes, such as Senna alexandrina. The effects of herbal medicine are linked with the solvent used. In making a herbal drink on an industrial and private scale, the solvent used is ethanol and water. This study aimed to determine the best solvent to produce antioxidant and antidiabetic effects from S. alexandrina leaves. In vitro analysis using DPPH and α-glucosidase enzyme was done to find the antioxidant and antidiabetic properties. S. alexandrina leaves were macerated using ethanol and water. All extract was screened for phytochemical contents, and the bioactive compounds were profiled using LC-MS/MS. Total phenolic and flavonoid contents were determined using gallic acid and quercetin as standard compounds. S. alexandrina leaves showed antioxidant and antidiabetic properties. Ethanol 50% extract showed the best antioxidant and antidiabetic properties, followed by ethanol and water extracts. The IC50 value of the antidiabetic activity of ethanol 50% was 33.151 µg/mL and the IC50 value of antioxidant power was 160.502 µg/mL. The bioactive compounds were profiled in this extract including torachrysone-8-O-β-D-glucopyranoside, oroxin B, 3-O-[β-D-glucopyra-nosyl-(12)]-β-D-glucopyranosyl-kaempferol, 7-hydroxy-1-methoxy-2-methoxyxanthone, rhamnetin dan rubilactone.

Kombucha is a fermented drink from herbs that provides many benefits for health, including antioxidant activity. Katuk (Sauropus androgynus (L.) Merr. ) leaves and kelor (Moringa oleifera Lam) leaves are common vegetables in Indonesia, and these plants were reported to contain natural antioxidants. This study aims to examine the antioxidant activity of single and mixture of katuk and kelor leaves before and after fermentation with Kombucha and identify the bioactive compounds of those samples. Katuk and kelor leaves were collected around the Serang Banten area. Antioxidant activity analysis was performed using free radicals DPPH method. Total phenolic content (TPC) and total flavonoids content (TFC) were measured. Compounds identification was conducted using liquid chromatography-mass spectrometry (LC-MS/MS). The results showed that the mixture of katuk and kelor fermented with Kombucha (KoKtKl 3:1) in the 50µl sample had the highest antioxidant activity (80.5% inhibition of DPPH), while under the same conditions, the unfermented sample only had 56.1% DPPH inhibition. Fermentation of katuk and kelor leaves with Kombucha for 7 days decreased pH, and increased antioxidant activity, TPC and TFC. The samples with the highest antioxidant activity (KoKtKl 3:1) were identified as containing kaempferol-3-O-rutinoside, kaempferol-3-O-β-D-glucopyranoside and quercimetrin, which are flavonoid glycosides that may be responsible for the antioxidant activity. In conclusion, katuk and kelor leaves showed antioxidant activity before and after fermentation with Kombucha, both as single preparations and mixtures which showed the potential to be studied further as a functional beverage for antioxidants.