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This work reports the phytochemical profile phenolic content and flavonoid, in vitro antioxidant against DPPH, s-carotene radical and total antioxidant activity of methanolic leaves extract from Tamarix gallica and Tamarix articulata. Formore, HPLC analysis for identification of individual polyphenols was identification in different phenolic groups and the quantification are also described. The quantification of total phenolic and flavonoids content realized by spectrophotometric methods. The antiradical capacities of extracts were evaluated against DPPH free radical and phosphomolybdenum method. Total phenolic contents and flavonoid from an extract of Tamarix gallica were founded high against Tamarix articulata, 334.19±8.47, 395.62±6.23 mg GAE/g DW for phenolic content and 159.73±6.28, 117.47±4.04 mg CE/g DW respectively. The HPLC analysis showed that at least 6 considerable phenolic compounds of leaves extract exist for the two extracts, the major ones being vanillic acid, naringin, and caffeic acid. Similar results for antioxidant activity leaves extract from Tamarix gallica exhibited strong inhibition of free radical against DPPH against Tamarix articulata IC50=5.70±0.18, 7.69±0.11 µg/ml and total antioxidant activity 341.69±9.28, 287.64±8.72 mg GAE/g DW respectively. The obtained results suggest that Tamarix gallica and Tamarix articulata could be a promising source for new drugs because of its antioxidant properties and phenolic compounds.