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Radboud University, 02 februari 2018, Promotor : Feitz, W.F.J. Co-promotores : Oosterwijk, E., Geutjes, P.J., Contains fulltext : 182801.pdf (publisher's version ) (Open Access)

Radboud University, 02 februari 2018, Promotor : Feitz, W.F.J. Co-promotores : Oosterwijk, E., Geutjes, P.J., Contains fulltext : 182801.pdf (publisher's version ) (Open Access)

Radboud University, 06 januari 2017, Promotor : Feitz, W.F.J. Co-promotores : Oosterwijk, E., Geutjes, P.J., Contains fulltext : 162524.pdf (publisher's version ) (Open Access)

Radboud University, 06 januari 2017, Promotor : Feitz, W.F.J. Co-promotores : Oosterwijk, E., Geutjes, P.J., Contains fulltext : 162524.pdf (publisher's version ) (Open Access)

25 mei 2016, Contains fulltext : 249346.pdf (Publisher’s version ) (Open Access), The invention relates to a process for making an oligo(alkylene glycol) functionalized co-polyisocyanopeptide, wherein the process comprises the steps of: i) copolymerizing - a first comonomer of an oligo(alkylene glycol) functionalized isocyanopeptide grafted with a linking group and - a second comonomer of a non-grafted oligo(alkylene glycol) functionalized isocyanopeptide, wherein the molar ratio between the first comonomer and the second comonomer is :500 and 1:30 and ii) adding a reactant of a spacer unit and a cell adhesion factor to the copolymer obtained by step i), wherein the spacer unit is represented by general formula A-L-B, wherein the linking group and group A are chosen to react and form a first coupling and the cell adhesion factor and group B are chosen to react and form a second coupling, wherein the first coupling and the second coupling are independently selected from the group consisting of alkyne-azide coupling, dibenzocyclooctyne-azide coupling, oxanorbornadiene-based-azide couplings, vinylsulphone-thiol coupling, maleimide-thiol coupling, methyl methacrylate-thiol coupling, ether coupling, thioether coupling, biotin- strepavidin coupling, amine-carboxylic acid resulting in amides linkages, alcohol- carboxylic acid coupling resulting in esters linkages and NHS-Ester (N- Hydroxysuccinimide ester)-amine coupling and wherein group L is a linear chain segment having 10-60 bonds between atoms selected from C, N, O and S in the main chain.

Radboud Universiteit Nijmegen, 11 juli 2014, Promotores : Feitz, W.F.J., Wijnen, R.H.M. Co-promotores : Geutjes, P.J., Kuppevelt, A.H.M.S.M. van, Daamen, W.F., Contains fulltext : 127375.pdf (publisher's version ) (Open Access), Severe congenital anomalies can have a large impact on the lives of patients. With tissue engineering techniques damaged or absent tissue can be regenerated, which can become a treatment option for congenital anomalies. In this thesis treatment possibilities for congenital anomalies concerning the bladder, for instance bladder exstrophy or myelomeningocele, and the abdominal wall, for instance gastroschisis, are described. A bladder exstrophy was surgically created in a fetal lamb, which largely resembled the anomaly in humans. In this model for diseased bladder we regenerated bladder tissue with a scaffold of bovine type I collagen, which served as an extracellular matrix. This tissue consisted of a urothelial layer, angiogenesis, smooth muscle cells and nerve fibers. The addition of growth factors improved angiogenesis, and the ingrowth of urothelium and smooth muscle cells. However, for a significant impact on clinical outcome, larger constructs need to be tested. A tubular collagen scaffold was combined with a polymer mesh and tested in a porcine model for its application as an incontinent urinary conduit, as an alternative for the current use of intestinal tissue. Although the construct served as a conduit in 50% of the animals, improvements need to be made before this technique can be studied in humans. In a fetal lamb model for gastroschisis the collagen scaffold was used to close the abdominal wall during the fetal period. Furthermore, we studied the coverage of the fetal bowel when it was left eviscerated outside the abdominal cavity. After birth less adhesions and fibrous peel was seen in the treated groups compared to the gastroschisis group. Skin and connective tissue with muscle cells replaced the scaffold. This treatment option may become an alternative for postnatal repair of abdominal wall defects when minimal invasive techniques (fetoscopy) improve, hereby decreasing the risk of premature delivery.

25 mei 2016, Contains fulltext : 249346.pdf (Publisher’s version ) (Open Access), The invention relates to a process for making an oligo(alkylene glycol) functionalized co-polyisocyanopeptide, wherein the process comprises the steps of: i) copolymerizing - a first comonomer of an oligo(alkylene glycol) functionalized isocyanopeptide grafted with a linking group and - a second comonomer of a non-grafted oligo(alkylene glycol) functionalized isocyanopeptide, wherein the molar ratio between the first comonomer and the second comonomer is :500 and 1:30 and ii) adding a reactant of a spacer unit and a cell adhesion factor to the copolymer obtained by step i), wherein the spacer unit is represented by general formula A-L-B, wherein the linking group and group A are chosen to react and form a first coupling and the cell adhesion factor and group B are chosen to react and form a second coupling, wherein the first coupling and the second coupling are independently selected from the group consisting of alkyne-azide coupling, dibenzocyclooctyne-azide coupling, oxanorbornadiene-based-azide couplings, vinylsulphone-thiol coupling, maleimide-thiol coupling, methyl methacrylate-thiol coupling, ether coupling, thioether coupling, biotin- strepavidin coupling, amine-carboxylic acid resulting in amides linkages, alcohol- carboxylic acid coupling resulting in esters linkages and NHS-Ester (N- Hydroxysuccinimide ester)-amine coupling and wherein group L is a linear chain segment having 10-60 bonds between atoms selected from C, N, O and S in the main chain.

Radboud Universiteit Nijmegen, 11 juli 2014, Promotores : Feitz, W.F.J., Wijnen, R.H.M. Co-promotores : Geutjes, P.J., Kuppevelt, A.H.M.S.M. van, Daamen, W.F., Contains fulltext : 127375.pdf (publisher's version ) (Open Access), Severe congenital anomalies can have a large impact on the lives of patients. With tissue engineering techniques damaged or absent tissue can be regenerated, which can become a treatment option for congenital anomalies. In this thesis treatment possibilities for congenital anomalies concerning the bladder, for instance bladder exstrophy or myelomeningocele, and the abdominal wall, for instance gastroschisis, are described. A bladder exstrophy was surgically created in a fetal lamb, which largely resembled the anomaly in humans. In this model for diseased bladder we regenerated bladder tissue with a scaffold of bovine type I collagen, which served as an extracellular matrix. This tissue consisted of a urothelial layer, angiogenesis, smooth muscle cells and nerve fibers. The addition of growth factors improved angiogenesis, and the ingrowth of urothelium and smooth muscle cells. However, for a significant impact on clinical outcome, larger constructs need to be tested. A tubular collagen scaffold was combined with a polymer mesh and tested in a porcine model for its application as an incontinent urinary conduit, as an alternative for the current use of intestinal tissue. Although the construct served as a conduit in 50% of the animals, improvements need to be made before this technique can be studied in humans. In a fetal lamb model for gastroschisis the collagen scaffold was used to close the abdominal wall during the fetal period. Furthermore, we studied the coverage of the fetal bowel when it was left eviscerated outside the abdominal cavity. After birth less adhesions and fibrous peel was seen in the treated groups compared to the gastroschisis group. Skin and connective tissue with muscle cells replaced the scaffold. This treatment option may become an alternative for postnatal repair of abdominal wall defects when minimal invasive techniques (fetoscopy) improve, hereby decreasing the risk of premature delivery.

25 mei 2016, Contains fulltext : 249346.pdf (Publisher’s version ) (Open Access), The invention relates to a process for making an oligo(alkylene glycol) functionalized co-polyisocyanopeptide, wherein the process comprises the steps of: i) copolymerizing - a first comonomer of an oligo(alkylene glycol) functionalized isocyanopeptide grafted with a linking group and - a second comonomer of a non-grafted oligo(alkylene glycol) functionalized isocyanopeptide, wherein the molar ratio between the first comonomer and the second comonomer is :500 and 1:30 and ii) adding a reactant of a spacer unit and a cell adhesion factor to the copolymer obtained by step i), wherein the spacer unit is represented by general formula A-L-B, wherein the linking group and group A are chosen to react and form a first coupling and the cell adhesion factor and group B are chosen to react and form a second coupling, wherein the first coupling and the second coupling are independently selected from the group consisting of alkyne-azide coupling, dibenzocyclooctyne-azide coupling, oxanorbornadiene-based-azide couplings, vinylsulphone-thiol coupling, maleimide-thiol coupling, methyl methacrylate-thiol coupling, ether coupling, thioether coupling, biotin- strepavidin coupling, amine-carboxylic acid resulting in amides linkages, alcohol- carboxylic acid coupling resulting in esters linkages and NHS-Ester (N- Hydroxysuccinimide ester)-amine coupling and wherein group L is a linear chain segment having 10-60 bonds between atoms selected from C, N, O and S in the main chain.

Radboud Universiteit Nijmegen, 15 september 2011, Promotores : Brock, R.E., Feitz, W.F.J. Co-promotores : Kuppevelt, A.H.M.S.M. van, Daamen, W.F., Geutjes, P.J., Contains fulltext : 97473.pdf (Publisher’s version ) (Open Access)

Radboud Universiteit Nijmegen, 15 september 2011, Promotores : Brock, R.E., Feitz, W.F.J. Co-promotores : Kuppevelt, A.H.M.S.M. van, Daamen, W.F., Geutjes, P.J., Contains fulltext : 97473.pdf (Publisher’s version ) (Open Access)

Radboud Universiteit Nijmegen, 11 november 2010, Promotor : Brock, R.E. Co-promotores : Kuppevelt, A.H.M.S.M. van, Daamen, W.F., Vliet, J.A. van der, Contains fulltext : 82964.pdf (publisher's version ) (Open Access)