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Kang Shuai Lao Pian (KSLP) is a traditional Chinese medicine (TCM) preparation used to delay aging. However, due to the lack of research on the chemical composition and pharmacokinetic behavior of KSLP, its material basis and in vivo components with high exposure remain ambiguous. The UPLC/Q-Orbitrap-MS/MS was performed to identify the preliminary chemical profile of KSLP. A total of 138 compounds, including ginsenosides, phenylethanol glycosides, iridoids, alkaloids, ionones and others, were identified in accordance with their retention times, accurate masses and characteristic MS/MS fragment patterns. Moreover, considering the active components and characteristic components of KSLP, the extraction process of KSLP was optimized, and the quantitative analysis by UPLC/QQQ-MS/MS of 13 compounds in KSLP was established. The method was stable and sensitive, and could be used for the quality control of KSLP. Then, the pharmacokinetic study was carried out by further refining the components of KSLP. Besides, quantitative method for 6 compounds in rat plasma was validated and developed by UPLC/QQQ-MS/MS. The established approach was successfully applied to characterize the pharmacokinetic features of components in KSLP and it was found that the absorption and elimination of ginsenosides in KSLP was slow. Altogether, this study laid a solid foundation and provided theoretical guidance for further clarification of bioactive components of KSLP.

Fluorescein isothiocyanate (FITC) was used for the fluorescent labeling of sweet corn cob polysaccharide (SCP), followed by identification using fluorescence spectroscopy, UV–Visible spectroscopy, infrared spectroscopy, and NMR analysis. The results confirmed the successful attachment of FITC to SCP with a substitution degree of 1.28%. This established fluorescent sweet corn cob polysaccharide (FSCP) was then subjected to an in vivo quantitative analysis, in which mice were administered with FSCP, fluorescent sweet corn cob polysaccharide nanoemulsion (FSCP-NE), and fluorescent sweet corn cob polysaccharide nano emulsion microcapsule (FSCP-NE-ME) via gavage followed by investigating the in vivo plasma pharmacokinetics and tissue distribution. The results revealed that FSCP-NE could improve the utilization efficiency of SCP, and the FSCP-NE microcapsules exhibited good controlled-release performance, which significantly improved the utilization efficiency of SCP. The absorption and metabolism of SCP in vivo were explored next, which provided the reference for future research and development on SCP for application in the fields of nutraceuticals and pharmaceuticals. [Display omitted] • Propose a pharmacokinetic study of the drug SCP. • The effect of nano-emulsions and microcapsules on drug absorption rate and slow release was studied. • Provides a reference for the application research and development of SCP in the fields of nutrition and pharmaceuticals. [ABSTRACT FROM AUTHOR]

Kang Shuai Lao Pian (KSLP) is a traditional Chinese medicine (TCM) preparation used to delay aging. However, due to the lack of research on the chemical composition and pharmacokinetic behavior of KSLP, its material basis and in vivo components with high exposure remain ambiguous. The UPLC/Q-Orbitrap-MS/MS was performed to identify the preliminary chemical profile of KSLP. A total of 138 compounds, including ginsenosides, phenylethanol glycosides, iridoids, alkaloids, ionones and others, were identified in accordance with their retention times, accurate masses and characteristic MS/MS fragment patterns. Moreover, considering the active components and characteristic components of KSLP, the extraction process of KSLP was optimized, and the quantitative analysis by UPLC/QQQ-MS/MS of 13 compounds in KSLP was established. The method was stable and sensitive, and could be used for the quality control of KSLP. Then, the pharmacokinetic study was carried out by further refining the components of KSLP. Besides, quantitative method for 6 compounds in rat plasma was validated and developed by UPLC/QQQ-MS/MS. The established approach was successfully applied to characterize the pharmacokinetic features of components in KSLP and it was found that the absorption and elimination of ginsenosides in KSLP was slow. Altogether, this study laid a solid foundation and provided theoretical guidance for further clarification of bioactive components of KSLP. [ABSTRACT FROM AUTHOR]