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Climate change is rapidly transforming Arctic landscapes where increasing soil temperatures speed up permafrost thaw. This exposes large carbon stocks to microbial decomposition, possibly worsening climate change by releasing more greenhouse gases. Understanding how microbes break down soil carbon, especially under the anaerobic conditions of thawing permafrost, is important to determine future changes. Here, we studied the microbial community dynamics and soil carbon decomposition potential in permafrost and active layer soils under anaerobic laboratory conditions that simulated an Arctic summer thaw. The microbial and viral compositions in the samples were analyzed based on metagenomes, metagenome-assembled genomes, and metagenomic viral contigs (mVCs). Following the thawing of permafrost, there was a notable shift in microbial community structure, with fermentative Firmicutes and Bacteroidota taking over from Actinobacteria and Proteobacteria over the 60-day incubation period. The increase in iron and sulfate-reducing microbes had a significant role in limiting methane production from thawed permafrost, underscoring the competition within microbial communities. We explored the growth strategies of microbial communities and found that slow growth was the major strategy in both the active layer and permafrost. Our findings challenge the assumption that fast-growing microbes mainly respond to environmental changes like permafrost thaw. Instead, they indicate a common strategy of slow growth among microbial communities, likely due to the thermodynamic constraints of soil substrates and electron acceptors, and the need for microbes to adjust to post-thaw conditions. The mVCs harbored a wide range of auxiliary metabolic genes that may support cell protection from ice formation in virus-infected cells.ImportanceAs the Arctic warms, thawing permafrost unlocks carbon, potentially accelerating climate change by releasing greenhouse gases. Our research delves into the underlying biogeochemical processes likely mediated by the soil microbial community in response to the wet and anaerobic conditions, akin to an Arctic summer thaw. We observed a significant shift in the microbial community post-thaw, with fermentative bacteria like Firmicutes and Bacteroidota taking over and switching to different fermentation pathways. The dominance of iron and sulfate-reducing bacteria likely constrained methane production in the thawing permafrost. Slow-growing microbes outweighed fast-growing ones, even after thaw, upending the expectation that rapid microbial responses to dominate after permafrost thaws. This research highlights the nuanced and complex interactions within Arctic soil microbial communities and underscores the challenges in predicting microbial response to environmental change.

Remote northern communities in Canada face energy challenges due to their reliance on fossil fuels. Small modular reactors (SMRs) show promise as a sustainable solution to this issue, as they can reduce greenhouse gas emissions and promote sustainable development. However, SMRs require specific structural and geotechnical design paradigms for implementation and operation in permafrost regions, which are adversely affected by climate change and permafrost degradation. This study presents the potential of SMRs for sustainable energy production in the northern context. We provide an overview of SMRs and highligh their position in Canada's energy transition in northern regions. Additionally, we discuss the challenges associated with designing and implementing SMRs in northern regions. Our study contributes to the growing body of literature on SMRs in permafrost regions and highlights the need for further research and policy development to support their adoption. [ABSTRACT FROM AUTHOR]

Metabolism in stream ecosystems alters the fate of organic carbon (OC) received from surrounding landscapes, but our understanding of in‐stream metabolic processes in boreal ecosystems remains limited. Determining the factors that regulate OC metabolism will help predict how the C balance of boreal streams may respond to future environmental change. In this study, we addressed the question: what controls OC metabolism in boreal headwater streams draining catchments with discontinuous permafrost? We hypothesized that metabolism is collectively regulated by OC reactivity, phosphorus availability, and temperature, with discharge modulating each of these conditions. We tested these hypotheses using a combination of laboratory experiments and whole‐stream ecosystem metabolism measurements throughout the Caribou‐Poker Creeks Research Watershed (CPCRW) in Interior Alaska, USA. In the laboratory experiments, respiration and dissolved OC (DOC) removal were both co‐limited by the supply of reactive C and phosphorus, but temperature and residence time acted as stronger controls of DOC removal. Ecosystem respiration (ER) was largely predicted by discharge and site, with some variance explained by gross primary production (GPP) and temperature. Both ER and GPP varied inversely with watershed permafrost extent, with an inverse relationship between temperature and permafrost extent providing one plausible explanation. Our results provide some of the first evidence of a functional response to permafrost thaw in stream ecosystems and suggest the role of metabolism in landscape C cycling may increase as climate change progresses. Plain Language Summary: Rivers and streams can transform organic matter received from the surrounding landscape through the metabolic activity of microbes and other organisms. In boreal streams, little is known about ecosystem metabolism and whether respiration rates might change as warming‐induced permafrost thaw progresses. To understand if and how permafrost thaw is affecting stream microbial activity, we compared respiration rates in streams draining areas with different amounts of permafrost and used lab experiments to test which factors are currently limiting microbial activity. We found that metabolic rates were greatest in streams where the most permafrost thaw had occurred. This pattern could be explained by warmer water temperature promoting faster rates of microbial activity and less flashy flow conditions allowing more time for microbes to break down organic matter before it is flushed downstream. This newly documented relationship between permafrost thaw and stream respiration should be considered in efforts to better incorporate in‐stream processes into broader carbon cycling models. Key Points: Stream ecosystem respiration increased with permafrost thaw, likely due to warmer temperature and a more stable flow regimeWithin individual streams, ecosystem respiration was positively correlated with both discharge and gross primary productionRespiration was most limited by temperature and residence time in lab experiments, with additional co‐limitation by carbon and phosphorus [ABSTRACT FROM AUTHOR]

The landscape and landforms in permafrost regions are transforming due to climate change and permafrost thaw. This study uses optical and radar remote sensing, alongside spatial analysis, to examine thermokarst features and their driving factors in the source region of the Yangtze River (SRYR) on the central Tibetan Plateau. We analyse the distribution, interaction, and key environmental factors influencing thermokarst ponds and ground surface deformation, which are the two widespread and noticeable thermokarst features. Since the 1960s, the number of small water bodies has doubled from approximately ~2 × 104 to ~4 × 104 by the 2020s, with the median size of these water bodies decreasing from 2.3 × 104 m2 to 1.4 × 104 m2. The permafrost terrain has an average subsidence rate of 6.8 mm/a. About 50.9% of the SRYR exhibits evident thermokarst features. Surficial geological factors, especially geomorphology and slope, are primary factors in shaping the spatial distributions of thermokarst features. Both seasonal deformation and long‐term subsidence rates are more pronounced in areas with thermokarst ponds. However, once pond coverage exceeds around 5%, the amplifying effect on long‐term subsidence rates and seasonal deformation diminishes. The investigation further reveals that the relationship between seasonal deformation and long‐term subsidence is not strictly linear and that the combined increase in seasonal deformation and long‐term subsidence applies only to areas with seasonal deformation below approximately 20 mm. Beyond this threshold, the long‐term subsidence rate is no longer exacerbated by increased seasonal deformation. [ABSTRACT FROM AUTHOR]

Our knowledge on permafrost carbon (C) cycle is crucial for understanding its feedback to climate warming and developing nature-based solutions for mitigating climate change. To understand the characteristics of permafrost C cycle on the Tibetan Plateau, the largest alpine permafrost region around the world, we summarized recent advances including the stocks and fluxes of permafrost C and their responses to thawing, and depicted permafrost C dynamics within this century. We find that this alpine permafrost region stores approximately 14.1 Pg (1 Pg=1015 g) of soil organic C (SOC) in the top 3 m. Both substantial gaseous emissions and lateral C transport occur across this permafrost region. Moreover, the mobilization of frozen C is expedited by permafrost thaw, especially by the formation of thermokarst landscapes, which could release significant amounts of C into the atmosphere and surrounding water bodies. This alpine permafrost region nevertheless remains an important C sink, and its capacity to sequester C will continue to increase by 2100. For future perspectives, we would suggest developing long-term in situ observation networks of C stocks and fluxes with improved temporal and spatial coverage, and exploring the mechanisms underlying the response of ecosystem C cycle to permafrost thaw. In addition, it is essential to improve the projection of permafrost C dynamics through in-depth model-data fusion on the Tibetan Plateau. [ABSTRACT FROM AUTHOR]

High-latitude warming is increasing soil temperatures and driving permafrost thaw, potentially altering soil nutrient conditions by enhancing microbial mineralization and making nutrients from previously frozen soils accessible for plant and microbial use. Increases in soil nutrient concentrations may alter plant community processes and, consequently, carbon (C) fluxes. We conducted an experiment in a boreal permafrost peatland, monitoring forest floor C flux and functional traits of the entire moss and vascular plant communities to the addition of nutrients at 20 and 40 cm soil depths and under closed and open canopy conditions. Plant functional trait responses were investigated at both community level (using community-weighted means) and intraspecific scales. Using fertilizer additions, we emulated nutrient increases at different depths in the soil profile, replicated at high and low canopy cover sites to assess the influence of light availability. Our results demonstrate rapid responses of vascular plant community-level traits as well as ecosystem respiration and gross primary productivity to fertilization treatments under low canopy cover, suggesting an influence of local environmental variation. We found that moss community-level traits played a more important role in mediating C flux response to nutrient fertilization than vascular plants but led to little change in C sink–source dynamics. This provides insight into existing ambiguities of the response of boreal C fluxes to increased nutrient availability following soil warming and permafrost thaw: Local environmental conditions and moss community can strongly mediate the response, whereas vascular plant communities may play a more minor role. However, our results suggest that these changes may not alter overall C sink–source dynamics of peatlands in the near term. [ABSTRACT FROM AUTHOR]

ABSTRACT Climate change is rapidly transforming Arctic landscapes where increasing soil temperatures speed up permafrost thaw. This exposes large carbon stocks to microbial decomposition, possibly worsening climate change by releasing more greenhouse gases. Understanding how microbes break down soil carbon, especially under the anaerobic conditions of thawing permafrost, is important to determine future changes. Here, we studied the microbial community dynamics and soil carbon decomposition potential in permafrost and active layer soils under anaerobic laboratory conditions that simulated an Arctic summer thaw. The microbial and viral compositions in the samples were analyzed based on metagenomes, metagenome-assembled genomes, and metagenomic viral contigs (mVCs). Following the thawing of permafrost, there was a notable shift in microbial community structure, with fermentative Firmicutes and Bacteroidota taking over from Actinobacteria and Proteobacteria over the 60-day incubation period. The increase in iron and sulfate-reducing microbes had a significant role in limiting methane production from thawed permafrost, underscoring the competition within microbial communities. We explored the growth strategies of microbial communities and found that slow growth was the major strategy in both the active layer and permafrost. Our findings challenge the assumption that fast-growing microbes mainly respond to environmental changes like permafrost thaw. Instead, they indicate a common strategy of slow growth among microbial communities, likely due to the thermodynamic constraints of soil substrates and electron acceptors, and the need for microbes to adjust to post-thaw conditions. The mVCs harbored a wide range of auxiliary metabolic genes that may support cell protection from ice formation in virus-infected cells.IMPORTANCEAs the Arctic warms, thawing permafrost unlocks carbon, potentially accelerating climate change by releasing greenhouse gases. Our research delves into the underlying biogeochemical processes likely mediated by the soil microbial community in response to the wet and anaerobic conditions, akin to an Arctic summer thaw. We observed a significant shift in the microbial community post-thaw, with fermentative bacteria like Firmicutes and Bacteroidota taking over and switching to different fermentation pathways. The dominance of iron and sulfate-reducing bacteria likely constrained methane production in the thawing permafrost. Slow-growing microbes outweighed fast-growing ones, even after thaw, upending the expectation that rapid microbial responses to dominate after permafrost thaws. This research highlights the nuanced and complex interactions within Arctic soil microbial communities and underscores the challenges in predicting microbial response to environmental change.

Rainfall frequency and intensity are expected to increase in the Arctic, with potential detrimental impacts on permafrost, leading to enhanced thawing and carbon release to the atmosphere. However, there have been very few studies on the effect of discrete rain events on permafrost in the Arctic and sub-Arctic. Conducting controlled rainfall experiments within permafrost landscapes can provide an improved understanding of the effect of changing intensity, duration, and timing of rain events on permafrost tundra ecosystems. Here, we describe the design and implementation of the Next-Generation Ecosystem Experiment Arctic Rainfall Simulator (NARS), a variable intensity (4–82 mm/h) rainfall simulator that can be used to study the effects of rainfall on permafrost stability. The NARS design includes a 3D-printed 4 cm H-flume and uses an eTape resistivity sensor that was calibrated (R2 = 0.9–0.96) to measure discharge from the system. NARS is designed to be lightweight, simple to construct, and can be easily deployed in remote locations. As a field validation of updated rainfall simulator design and modernized controls, NARS was tested on the Seward Peninsula, AK. Because of its portability, versatility in deployment, dimensions, and rainfall intensity, NARS represents a methodological innovation for researching the impacts of rainfall on permafrost environments. HIGHLIGHTS Rainfall is expected to increase in the Arctic over the next century.; The effects of rainfall on permafrost stability are poorly understood.; We developed a variable intensity rainfall simulator for use in remote areas.; The new rainfall simulator is lightweight and modernizes simulator controls.; The simulator can be used to study rain effects on permafrost.;

Permafrost soils in the northern hemisphere are known to harbor large amounts of soil organic matter (SOM). Global climate warming endangers this stable soil organic carbon (SOC) pool by triggering permafrost thaw and deepening the active layer, while at the same time progressing soil formation. But depending, e.g., on ice content or drainage, conditions in the degraded permafrost can range from water-saturated/anoxic to dry/oxic, with concomitant shifts in SOM stabilizing mechanisms. In this field study in Interior Alaska, we investigated two sites featuring degraded permafrost, one water-saturated and the other well-drained, alongside a third site with intact permafrost. Soil aggregate- and density fractions highlighted that permafrost thaw promoted macroaggregate formation, amplified by the incorporation of particulate organic matter, in topsoils of both degradation sites, thus potentially counteracting a decrease in topsoil SOC induced by the permafrost thawing. However, the subsoils were found to store notably less SOC than the intact permafrost in all fractions of both degradation sites. Our investigations revealed up to net 75% smaller SOC storage in the upper 100 cm of degraded permafrost soils as compared to the intact one, predominantly related to the subsoils, while differences between soils of wet and dry degraded landscapes were minor. This study provides evidence that the consideration of different permafrost degradation landscapes and the employment of soil fractionation techniques is a useful combination to investigate soil development and SOM stabilization processes in this sensitive ecosystem. [ABSTRACT FROM AUTHOR]

Rainfall frequency and intensity are expected to increase in the Arctic, with potential detrimental impacts on permafrost, leading to enhanced thawing and carbon release to the atmosphere. However, there have been very few studies on the effect of discrete rain events on permafrost in the Arctic and sub-Arctic. Conducting controlled rainfall experiments within permafrost landscapes can provide an improved understanding of the effect of changing intensity, duration, and timing of rain events on permafrost tundra ecosystems. Here, we describe the design and implementation of the Next-Generation Ecosystem Experiment Arctic Rainfall Simulator (NARS), a variable intensity (4-82mm/h) rainfall simulator that can be used to study the effects of rainfall on permafrost stability. The NARS design includes a 3D-printed 4 cm H-flume and uses an eTape resistivity sensor that was calibrated (R2? 0.9-0.96) to measure discharge from the system. NARS is designed to be lightweight, simple to construct, and can be easily deployed in remote locations. As a field validation of updated rainfall simulator design and modernized controls, NARS was tested on the Seward Peninsula, AK. Because of its portability, versatility in deployment, dimensions, and rainfall intensity, NARS represents a methodological innovation for researching the impacts of rainfall on permafrost environments. [ABSTRACT FROM AUTHOR]

Climate warming holds the potential to cause extensive drying of wetlands in the Arctic, but the warming-drying effects on belowground ecosystems, particularly micro-eukaryotes, remain poorly understood. We investigated the responses of soil micro-eukaryotic communities, including fungi, protists, and microbial metazoa, to decadal drainage manipulation in a Siberian wet tundra using both amplicon and shotgun metagenomic sequencing. Our results indicate that drainage treatment increased the abundance of both fungal and non-fungal micro-eukaryotic communities, with key groups such as Ascomycota (mostly order Helotiales), Nematoda, and Tardigrada being notably abundant in drained sites. Functional traits analysis showed an increase in litter saprotrophic fungi and protistan consumers, indicating their increased activities in drained sites. The effects of drainage were more pronounced in the surface soil layer than the deeper layer, as soils dry and warm from the surface. Marked compositional shifts were observed for both communities, with fungal communities being more strongly influenced by drainage-induced vegetation change than the lowered water table itself, while the vegetation effect on non-fungal micro-eukaryotes was moderate. These findings provide insights into how belowground micro-eukaryotic communities respond to the widespread drying of wetlands in the Arctic and improve our predictive understanding of future ecosystem changes.

Permafrost regions, characterised by extensive belowground excess ice, are highly vulnerable to rapid thaw, particularly in areas such as the Yedoma domain. This region is known to freeze-lock a globally significant stock of soil nitrogen (N). However, the fate of this N upon permafrost thaw remains largely unknown. In this study, we assess the impact of climate warming on the size and dynamics of the soil N pool in (sub-)Arctic ecosystems, drawing upon recently published data and literature. Our findings suggest that climate warming and increased thaw depths will result in an expansion of the reactive soil N pool due to the larger volume of (seasonally) thawed soil. Dissolved organic N emerges as the predominant N form for rapid cycling within (sub-)Arctic ecosystems. The fate of newly thawed N from permafrost is primarily influenced by plant uptake, microbial immobilisation, changes in decomposition rates due to improved N availability, as well as lateral flow. The Yedoma domain contains substantial N pools, and the partial but increasing thaw of this previously frozen N has the potential to amplify climate feedbacks through additional nitrous oxide (N _2 O) emissions. Our ballpark estimate indicates that the Yedoma domain may contribute approximately 6% of the global annual rate of N _2 O emissions from soils under natural vegetation. However, the released soil N could also mitigate climate feedbacks by promoting enhanced vegetation carbon uptake. The likelihood and rate of N _2 O production are highest in permafrost thaw sites with intermediate moisture content and disturbed vegetation, but accurately predicting future landscape and hydrology changes in the Yedoma domain remains challenging. Nevertheless, it is evident that the permafrost-climate feedback will be significantly influenced by the quantity and mobilisation state of this unconsidered N pool.

Climate change is rapidly transforming Arctic landscapes where increasing soil temperatures speed up permafrost thaw. This exposes large carbon stocks to microbial decomposition, possibly worsening climate change by releasing more greenhouse gases. Understanding how microbes break down soil carbon, especially under the anaerobic conditions of thawing permafrost, is important to determine future changes. Here, we studied the microbial community dynamics and soil carbon decomposition potential in permafrost and active layer soils under anaerobic laboratory conditions that simulated an Arctic summer thaw. The microbial and viral compositions in the samples were analyzed based on metagenomes, metagenome-assembled genomes, and metagenomic viral contigs (mVCs). Following the thawing of permafrost, there was a notable shift in microbial community structure, with fermentative Firmicutes and Bacteroidota taking over from Actinobacteria and Proteobacteria over the 60-day incubation period. The increase in iron and sulfate-reducing microbes had a significant role in limiting methane production from thawed permafrost, underscoring the competition within microbial communities. We explored the growth strategies of microbial communities and found that slow growth was the major strategy in both the active layer and permafrost. Our findings challenge the assumption that fast-growing microbes mainly respond to environmental changes like permafrost thaw. Instead, they indicate a common strategy of slow growth among microbial communities, likely due to the thermodynamic constraints of soil substrates and electron acceptors, and the need for microbes to adjust to post-thaw conditions. The mVCs harbored a wide range of auxiliary metabolic genes that may support cell protection from ice formation in virus-infected cells., Importance: As the Arctic warms, thawing permafrost unlocks carbon, potentially accelerating climate change by releasing greenhouse gases. Our research delves into the underlying biogeochemical processes likely mediated by the soil microbial community in response to the wet and anaerobic conditions, akin to an Arctic summer thaw. We observed a significant shift in the microbial community post-thaw, with fermentative bacteria like Firmicutes and Bacteroidota taking over and switching to different fermentation pathways. The dominance of iron and sulfate-reducing bacteria likely constrained methane production in the thawing permafrost. Slow-growing microbes outweighed fast-growing ones, even after thaw, upending the expectation that rapid microbial responses to dominate after permafrost thaws. This research highlights the nuanced and complex interactions within Arctic soil microbial communities and underscores the challenges in predicting microbial response to environmental change., Competing Interests: The authors declare no conflict of interest.

Climate warming holds the potential to cause extensive drying of wetlands in the Arctic, but the warming-drying effects on belowground ecosystems, particularly micro-eukaryotes, remain poorly understood. We investigated the responses of soil micro-eukaryotic communities, including fungi, protists, and microbial metazoa, to decadal drainage manipulation in a Siberian wet tundra using both amplicon and shotgun metagenomic sequencing. Our results indicate that drainage treatment increased the abundance of both fungal and non-fungal micro-eukaryotic communities, with key groups such as Ascomycota (mostly order Helotiales), Nematoda, and Tardigrada being notably abundant in drained sites. Functional traits analysis showed an increase in litter saprotrophic fungi and protistan consumers, indicating their increased activities in drained sites. The effects of drainage were more pronounced in the surface soil layer than the deeper layer, as soils dry and warm from the surface. Marked compositional shifts were observed for both communities, with fungal communities being more strongly influenced by drainage-induced vegetation change than the lowered water table itself, while the vegetation effect on non-fungal micro-eukaryotes was moderate. These findings provide insights into how belowground micro-eukaryotic communities respond to the widespread drying of wetlands in the Arctic and improve our predictive understanding of future ecosystem changes., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Myeong, Kwon, Göckede, Tripathi and Kim.)