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Your search keyword '"de Villena, Fernando Pardo-Manuel"' showing total 9 results
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9 results on '"de Villena, Fernando Pardo-Manuel"'

4. Genetic loci regulate Sarbecovirus pathogenesis: A comparison across mice and humans

Author

Schäfer, Alexandra, Gralinski, Lisa E., Leist, Sarah R., Hampton, Brea K., Mooney, Michael A., Jensen, Kara L., Graham, Rachel L., Agnihothram, Sudhakar, Jeng, Sophia, Chamberlin, Steven, Bell, Timothy A., Scobey, D. Trevor, Linnertz, Colton L., VanBlargan, Laura A., Thackray, Larissa B., Hock, Pablo, Miller, Darla R., Shaw, Ginger D., Diamond, Michael S., de Villena, Fernando Pardo Manuel, McWeeney, Shannon K., Heise, Mark T., Menachery, Vineet D., Ferris, Martin T., and Baric, Ralph S.

Published
2024
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5. Mapping of susceptibility loci for Ebola virus pathogenesis in mice

Author

Schäfer, Alexandra, Marzi, Andrea, Furuyama, Wakako, Catanzaro, Nicholas J., Nguyen, Cameron, Haddock, Elaine, Feldmann, Friederike, Meade-White, Kimberly, Thomas, Tina, Hubbard, Miranda L., Gully, Kendra L., Leist, Sarah R., Hock, Pablo, Bell, Timothy A., De la Cruz, Gabriela E., Midkiff, Bentley R., Martinez, David R., Shaw, Ginger D., Miller, Darla R., Vernon, Michael J., Graham, Rachel L., Cowley, Dale O., Montgomery, Stephanie A., Schughart, Klaus, de Villena, Fernando Pardo Manuel, Wilkerson, Gregory K., Ferris, Martin T., Feldmann, Heinz, and Baric, Ralph S.

Published
2024
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8. Large changes in detected selection signatures after a selection limit in mice bred for voluntary wheel-running behavior.

Author

Hillis, David A., Yadgary, Liran, Weinstock, George M., de Villena, Fernando Pardo-Manuel, Pomp, Daniel, and Garland Jr., Theodore

Abstract

In various organisms, sequencing of selectively bred lines at apparent selection limits has demonstrated that genetic variation can remain at many loci, implying that evolution at the genetic level may continue even if the population mean phenotype remains constant. We compared selection signatures at generations 22 and 61 of the "High Runner" mouse experiment, which includes 4 replicate lines bred for voluntary wheel-running behavior (HR) and 4 non-selected control (C) lines. Previously, we reported multiple regions of differentiation between the HR and C lines, based on whole-genome sequence data for 10 mice from each line at generation 61, which was >31 generations after selection limits had been reached in all HR lines. Here, we analyzed pooled sequencing data from ~20 mice for each of the 8 lines at generation 22, around when HR lines were reaching limits. Differentiation analyses of allele frequencies at ~4.4 million SNP loci used the regularized T-test and detected 258 differentiated regions with FDR = 0.01. Comparable analyses involving pooling generation 61 individual mouse genotypes into allele frequencies by line produced only 11 such regions, with almost no overlap among the largest and most statistically significant peaks between the two generations. These results implicate a sort of "genetic churn" that continues at loci relevant for running. Simulations indicate that loss of statistical power due to random genetic drift and sampling error are insufficient to explain the differences in selection signatures. The 13 differentiated regions at generation 22 with strict culling measures include 79 genes related to a wide variety of functions. Gene ontology identified pathways related to olfaction and vomeronasal pathways as being overrepresented, consistent with generation 61 analyses, despite those specific regions differing between generations. Genes Dspp and Rbm24 are also identified as potentially explaining known bone and skeletal muscle differences, respectively, between the linetypes. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Early hippocampal high-amplitude rhythmic spikes predict post-traumatic epilepsy in mice.

Author

Shannon T, Levine N, Dirickson R, Shen Y, Cotter C, Yi YJ, Rajjoub N, de Villena FP, Kokiko-Cochran O, and Gu B

Abstract

Oscillations, a highly conserved brain function across mammalian species, are pivotal in brain physiology and pathology. Traumatic brain injury (TBI) often leads to subacute and chronic brain oscillatory alterations associated with complications like post-traumatic epilepsy (PTE) in patients and animal models. Our recent work longitudinally recorded local field potential from the contralateral hippocampus of 12 strains of recombinant inbred Collaborative Cross (CC) mice alongside classical laboratory inbred C57BL/6J mice after lateral fluid percussion injury. In this study, we profiled the acute (<12 hr post-injury) and subacute (12-48 hr post-injury) hippocampal oscillatory responses to TBI and evaluated their predictive value for PTE. We found dynamic high-amplitude rhythmic spikes with elevated power density and reduced entropy that prevailed during the acute phase in CC031 mice who later developed PTE. This characteristic early brain oscillatory alteration is absent in CC031 sham controls or other CC and reference C57BL/6J strains that did not develop PTE after TBI. Our work provides quantitative measures linking early brain oscillation to PTE at a population level in mice under controlled experimental conditions. These findings will offer insights into circuit mechanisms and potential targets for neuromodulatory intervention., Competing Interests: Authors’ disclosure statement: Neither of the authors has any conflict of interest to disclose.

Published
2024
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