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The gadolinium-based contrast agent DOTA-Gd is clinically used in combination with local anesthetics for direct magnetic resonance arthrography. It remains unclear whether gadolinium uptake into cartilage is influenced by co-administration of bupivacaine or ropivacaine and whether DOTA-Gd alters their chondrotoxicity. Gadolinium quantification of chondrogenic spheroids revealed enhanced gadolinium uptake after simultaneous exposure to local anesthetics. Analyses of the spatial gadolinium distribution using synchrotron X-ray-fluorescence scanning indicates gadolinium exposed chondrocytes. In vitro exposure to DOTA-Gd does not alter viability and proliferation of human chondrocytes and the chondrotoxic potential of the anesthetics. Reduced viability induced by ropivacaine was found to be reversible, while exposure to bupivacaine leads to irreversible cell death. Our data suggest that ropivacaine is more tolerable than bupivacaine and that DOTA-Gd exposure does not alter the cytotoxicity of both anesthetics. Enhanced gadolinium uptake into cartilage due to co-administration of anesthetics should find attention.

Background: During articulation the velopharynx needs to be opened and closed rapidly and a tight closure is needed. Based on the hypothesis that patients with cleft lip and palate (CLP) produce lower pressures in the velopharynx than healthy individuals, this study compared pressure profiles of the velopharyngeal closure during articulation of different sounds between healthy participants and patients with surgically closed unilateral CLP (UCLP) using high resolution manometry (HRM). Materials and methods: Ten healthy adult volunteers (group 1: 20–25.ears) and ten patients with a non-syndromic surgically reconstructed UCLP (group 2: 19.1–26.9 years) were included in this study. Pressure profiles during the articulation of four sounds (/i:/, /s/, /ʃ/ and /n/) were measured by HRM. Maximum, minimum and average pressures, time intervals as well as detection of a previously described 3phase-model were compared.Results: Both groups presented with similar pressure curves for each phoneme with regards to the phases described and pressure peaks, but differed in total pressures. An exception was noted for the sound /i:/, where a 3-phase-model could not be seen for most patients with UCLP. Differences in velopharynx pressures of 50% and more were found between the two groups. Maximum and average pressures in the production of the alveolar fricative reached statistical significance.Conclusions: It can be concluded that velopharyngeal pressures of patients with UCLP are not sufficient to eliminate nasal resonance or turbulence during articulation, especially for more complex sounds. These results support a general understanding of hypernasality during speech implying a (relative) velopharyngeal insufficiency.

Background: Medial patellofemoral ligament reconstruction (MPFL-R) aims to restore proper ligament function with minimal changes in length during range of motion, yet the ideal area for femoral fixation of the graft remains controversial. Purpose: To determine the region where the isometric circular path of a simulated MPFL graft (best-fit circle) follows the sagittal radius curvature of the trochlea in normal (nontrochlear dysplastic) knees and to evaluate the best-fit circle coverage of different femoral fixation points in knees with severe trochlear dysplasia (TD) and after deepening trochleoplasty. Study Design: Controlled laboratory study. Methods: Twelve patients (4 male, 8 female; mean age, 24 ± 8 years) who underwent surgical treatment for recurrent lateral patellar instability due to severe TD were prospectively enrolled in this study. Four previously defined reference points for the femoral MPFL-R (Schöttle, Fujino, Stephen, and Oka) were identified, and the best-fit circle was drawn along the sagittal trochlear groove curvature. The divergence between each best-fit circle and the trochlear groove was calculated, with negative values indicating relative slackening and positive values indicating relative tightening of the simulated MPFL graft. Measurements were made on true-lateral fluoroscopic images before and after deepening trochleoplasty and compared with those of a sex-matched control group. Results: The best-fit circle of the Schöttle point followed the sagittal curvature of the trochlea most closely in both the control and trochlear dysplastic knees, followed by the Fujino, Stephen, and Oka points. As the radius of the trochlear groove curvature increased, the divergence of all best-fit circles to the trochlear groove became negative (all P <.05). This effect was most pronounced at the Stephen and Oka points, followed by the Fujino and Schöttle points (all P <.05). After deepening trochleoplasty, the divergence of the Schöttle point changed toward positive values (11.6% at 40°; P <.001). Concurrently, the best-fit circle divergence of all other reference points improved toward baseline (all P <.05). Conclusion: The isometric circle of the Schöttle point provides the best congruence with the sagittal trochlear groove curvature in both the normal trochlea and the dysplastic trochlea. After trochleoplasty, the best-fit circles of more distal femoral fixation points resulted in better congruence with the deepened trochlear groove, whereas the best-fit circle of Schöttle indicated graft tension during flexion. Clinical Relevance: According to the present study, different femoral fixation points should be considered depending on whether the TD is corrected. [ABSTRACT FROM AUTHOR]

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Fuel cells used for transport applications hold polymer membranes as a key element. Their efficiency can be significantly increased if structured ion channels are implemented at the molecular level into the proton‐conducting membrane. New functional molecules with selective affinity for ionomers are needed to obtain such a membrane design. This study presents a method to screen for selective peptide binders to perfluorinated sulfonic acid ionomers, e.g., Nafion using ultra‐high density peptide arrays with a spot size of up to 30 µm. First, the ionomer dispersion is incubated with the peptide chip containing 56014 randomly chosen 6‐mer peptides. Afterward, the peptide chip is washed. The peptide WIWHCW with the helix structure is identified as a selective binder to Nafion. The invariant amino acids responsible for binding are also determined using a peptide chip approach. The specific binding pocket of WIWHCW is formed by histidine and tryptophan. Its dissociation constant to the ionomer is measured at ≈140 µM. [ABSTRACT FROM AUTHOR]

The long-term (AD 1300–1800), multi-scale interactions of monocropping and subaltern agri-food systems of Andalus (Spain) and coastal Peru reveal the entangled transformations of the Plantationocene. Historically convergent colonial monocultures (wheat, sugar, cotton, sheep, cattle) entangled with the divergence and plurality of resilient yet precarious diverse-food affordances of subaltern groups (peasants, indigenous people, enslaved persons, Mudejares, Moriscos). Using political ecology, the comparative cases illuminate how Plantationocene colonial entanglements were shaped through spatial movements and social-environmental affordances of biota, populations, and institutions. New empirical understanding and a novel conceptual and analytical framework offer insight into Plantationocene pathways, alternatives, and struggles. [ABSTRACT FROM AUTHOR]

New 40Ar/39Ar ages, combined with selected ages from published studies, provide detailed insight into the late Quaternary (<500 ka) eruptive history and related hazards of the Rio Grande rift and Jemez lineament of New Mexico, USA. Most eruptions in the region during this time were within monogenetic volcanic fields, which largely produced cinder cones and mafic lava flows. 40Ar/39Ar ages of mafic groundmass determined using the high-sensitivity ARGUS VI multicollector mass spectrometer are significantly more precise, by as much as an order of magnitude, than prior 40Ar/39Ar dates. The high-precision data permit more rigorous interpretation of age spectra and isochrons, leading to a greater confidence in assigning eruption ages, and thus allowing more accurate and thorough calculations of eruptive rates and repose periods. For most fields, comprehensive dating identifies a greater number of late Quaternary eruptions than previously known and, for some fields, determines younger-than-previously established ages for the last eruptive events. Repose periods in the fields range from too short to measure with the 40Ar/39Ar method to a few hundred thousand years, which suggests that all 12 fields in the rift and lineament with late Quaternary activity should be considered dormant rather than extinct, with the possibility of future eruptions. Average recurrence intervals for these fields during the late Quaternary range from 16.5 k.y. to 170.8 k.y. Many fields display peak periods of activity where rates spike to a recurrence interval of 5 k.y. or less. At the scale of the entire rift and lineament, 75 late Quaternary eruptions were dated, yielding an average recurrence interval of 6.5 k.y., which is a minimum estimate considering the likelihood of undated eruptions (either not studied or buried). During the last 100 k.y., the volcanic record is better preserved, and the recurrence interval is 3.2 k.y., which indicates that the current hiatus of 3.9 ± 1.2 k.y. is typical for the region. Since ca. 36 ka, the average recurrence interval decreased to 2.3 k.y., which suggests a slight increase in recent activity. When ages are compared to vent locations, a previously unrecognized migrational pattern is observed in nearly all of the fields studied. Migration vectors vary from 1.0 cm/yr to 4.0 cm/yr, and always with an eastward component--similar to migration patterns at some other late Cenozoic fields throughout the American Southwest. Volcanic migration is attributed to a combination of mechanisms including asthenospheric convection along the margin of the Colorado Plateau, North American plate motion over partial mantle melt, and extensional tectonics. Developing similar high-precision chronologies for other Quaternary fields throughout southwestern North America will be necessary to better understand the volcanic hazards of the region. [ABSTRACT FROM AUTHOR]

The article explores how ethics committees worldwide review biomedical research proposals, emphasizing both individual protections and societal implications. It discusses how different countries' regulations either require, permit, or prohibit consideration of societal and long-term impacts in research ethics reviews. It suggests potential innovations like embedding multidisciplinary scholars to better address these complex ethical issues early in the research process.

Purpose: The aim of this prospective cohort study was to assess the factual accuracy, completeness of medical information, and potential harmfulness of incorrect conclusions by medical professionals in automatically generated texts of varying complexity (1) using ChatGPT, Furthermore, patients without a medical background were asked to evaluate comprehensibility, information density, and conclusion possibilities (2). Methods: In the study, five different simplified versions of MRI findings of the knee of different complexity (A: simple, B: moderate, C: complex) were each created using ChatGPT. Subsequently, a group of four medical professionals (two orthopedic surgeons and two radiologists) and a group of 20 consecutive patients evaluated the created reports. For this purpose, all participants received a group of simplified reports (simple, moderate, and severe) at intervals of 1 week each for their respective evaluation using a specific questionnaire. Each questionnaire consisted of the original report, the simplified report, and a series of statements to assess the quality of the simplified reports. Participants were asked to rate their level of agreement with a five-point Likert scale. Results: The evaluation of the medical specialists showed that the findings produced were consistent in quality depending on their complexity. Factual correctness, reproduction of relevant information and comprehensibility for patients were rated on average as "Agree". The question about possible harm resulted in an average of "Disagree". The evaluation of patients also revealed consistent quality of reports, depending on complexity. Simplicity of word choice and sentence structure was rated "Agree" on average, with significant differences between simple and complex findings (p = 0.0039) as well as between moderate and complex findings (p = 0.0222). Participants reported being significantly better at knowing what the text was about (p = 0.001) and drawing the correct conclusions the more simplified the report of findings was (p = 0.013829). The question of whether the text informed them as well as a healthcare professional was answered as "Neutral" across all findings. Conclusion: By using ChatGPT, MRI reports can be simplified automatically with consistent quality so that the relevant information is understandable to patients. However, a report generated in this way does not replace a thorough discussion between specialist and patient. [ABSTRACT FROM AUTHOR]

Our newly acquired and recently published map, geochronologic, and compositional data for early intermediate-composition central volcanoes in the northeastern San Juan Mountains provide insights about the broad magmatic precursors to the large continental-arc ignimbrite flare-up in the mid-Cenozoic Southern Rocky Mountain volcanic field (SRMVF). Initial volcanism migrated from central Colorado to northern New Mexico ca. 40-29 Ma, as part of a more regional trend of southward-progressing mid-Cenozoic magmatism in the U.S. segment of the North American Cordillera. Within the San Juan locus, which represents the largest preserved erosional remnant of the SRMVF and site of most intense eruptive activity, new 40Ar/39Ar and U-Pb zircon ages show that eruptions at many individual edifices began nearly concurrently, at ca. 35 Ma, with peak activity at 34-32 Ma. Broadly similar precursor effusive volcanism characterizes other major loci of continental-arc ignimbrite magmatism along the western American cordilleras, but none of these sites records early volcanism as voluminous, spatially widespread, well exposed, or compositionally diverse as the San Juan locus in Colorado. Early San Juan volcanism was larger in volume than the later ignimbrites, constituting about two thirds the total erupted. Early lava and breccias are as much as 700-900 m thick where exposed along eroded flanks of the San Juan Mountains; drill holes have penetrated sections as thick as 2600 m. The early volcanoes were dominantly andesitic, with lesser dacite and minor rhyolite. Such volcanism is widely interpreted as initiated by basaltic magma from the mantle, but mid-Cenozoic basalt is almost nonexistent at the San Juan locus--an absence inferred to be due to extensive lower-crustal assimilation and fractionation. The early volcanic rocks are calc-alkaline and typical of high-K continental-arc volcanism; they become modestly more alkalic and enriched in trace elements such as light rare earth elements, Zr, Nb, and Th from the San Juan locus northeastward into central Colorado. Such variations may reflect synmagmatic crustal thickening and deeper levels of primary magma generation concurrent with assembly of upper-crustal magma bodies that could support large ignimbrite eruptions. Substantial uncertainties remain for growth histories of the early volcanoes, however, because of unexposed lower parts of edifices, eroded upper parts, and limited availability of mineral phases that could be dated reliably. Although the early volcanoes are widely distributed within the SRMVF, many are clustered at sites of subsequent ignimbrite calderas. The precursor edifices are inferred to record incubation stages in construction of overall translithospheric batholithic-scale magmatic systems. Prolonged processes of incremental magma generation, accumulation, fractionation, and solidification intermittently generated sufficient liquid to erupt large ignimbrites. Maturation of focused eruptions and intrusions was prolonged, 5 m.y. or more, prior to the culminating ignimbrite at some centers in the San Juan Mountains. Some large-volume ignimbrites and related calderas, including the ~5000 km3 Fish Canyon Tuff and associated La Garita caldera, formed as much as several million years later than peak growth of associated precursor volcanoes, recording a sustained interval of diminished eruptive activity as the magma reservoir increased in volume and evolved to more silicic compositions capable of supporting a subsequent large ignimbrite eruption. Dike configurations at early volcanoes that were active nearly concurrently in the SRMVF vary from symmetrically radial to more parallel trends. The contrasting dike geometries are inferred to record possible multiple fluctuations from compressive to weakly extensional regional stress, concurrent with destabilization of the prior flat-slab plate configuration that triggered mid-Cenozoic ignimbrite flare-ups along the Cordilleran margin of the North American plate. These apparent fluctuations in regional stress preceded development of substantial extensional strain in the Southern Rocky Mountain region; outflow ignimbrite sheets of the SRMVF spread across subsequent horst-and-graben structures of the Rio Grande rift without complementary thickness variations. [ABSTRACT FROM AUTHOR]

Polyaryletherketones (PAEK) are high-performance plastics with excellent mechanical properties and high thermal resistance used, e.g. as material for implants. Their molecular structure gives them a high level of chemical inertness but prevents bonding and adhesive interactions with other materials and integration into body tissue. Using the example of poly(ether ether ketone) (PEEK), we show novel synthetic routes for wet-chemical modification of the surface to create reactive carboxylic and amino groups. In a first approach, the ketone functionalities of the PEEK molecules were reduced to hydroxy groups and subsequently esterified with carboxylic acids. Using dicarboxylic acids, one acid group was bonded covalently to the PEEK surface while the other provided free acidic functionalities. In a second approach, the PEEK surface was modified in one step by reaction of the ketone group with a diamine, creating primary amino groups on the PEEK surface. All reaction products were analyzed by a powerful combination of surface sensitive X-ray photoelectron spectroscopy (XPS) and advanced infrared spectroscopic methods. [ABSTRACT FROM AUTHOR]