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Step‐up variations are frequently used in sports performance to develop coordinated and powerful movements that transfer to running. This study aimed to quantify the kinetic characteristics of the first foot contact of four different step‐up variations. Ten professional rugby league players participated in this study and performed the Barbell One Box Step‐Up with Catch (BB1), Barbell Two Box Step‐Up (BB2), Vest Two Box Run (VEST) and Step‐Up Jump (JUMP) as part of routine in‐season strength training sessions during one season. Peak force, total impulse and maximal rate of force development (RFD) were measured from first foot contact on the step‐up box. Significantly greater peak force and RFD were observed in JUMP than any other variation (standardized mean difference; SMD: 3.9–5.5; p < 0.001). Total impulse was equal between JUMP and BB1, and significantly greater in JUMP than BB2 and VEST (SMD: 1.3–2.3; p < 0.001), and in BB1 than BB2 and VEST (SMD: 1.8–2.8; p < 0.001). Significantly larger peak force and RFD were observed in BB2 and VEST than BB1 (SMD: 0.6–0.7) and in total impulse in BB2 than VEST (SMD: 1.6) (p < 0.05). The results of this study highlight that step‐up exercise variations maximize different kinetic characteristics, which may transfer differently to athlete running performance. Highlights: The Step‐Up Jump variation was shown to produce the largest relative peak force, largest rate of force development (RFD) and equal largest total impulse and therefore may have the greatest transferability to maximal speed running through the maximization of the stance phase.The Barbell Two Box Step‐Up (BB2) and Vest Two Box Run (VEST), produced significantly greater peak relative force and RFD than the Barbell One Box Step‐Up with Catch (BB1) and therefore may have better transferability to acceleration ability.Coaches can use the findings of this study to assist their selection of step‐up variations that are best suited to develop running ability and should consider the testing results and individual requirements of each athlete. [ABSTRACT FROM AUTHOR]