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Your search keyword '"Wabnik, Krzysztof"' showing total 4 results
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4 results on '"Wabnik, Krzysztof"'

1. Core clock genes adjust growth cessation time to day-night switches in poplar

Author

Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Ministerio de Universidades (España), Alique, Daniel [0000-0001-7869-9411], Redondo-López, Arturo [0000-0003-2037-5400], González Schain, Nahuel [0000-0003-2446-5825], Allona, Isabel [0000-0002-7012-2850], Wabnik, Krzysztof [0000-0001-7263-0560], Perales, Mariano [0000-0002-7351-8439], Alique, Daniel, Redondo-López, Arturo, González Schain, Nahuel, Allona, Isabel, Wabnik, Krzysztof, Perales, Mariano, Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Ministerio de Universidades (España), Alique, Daniel [0000-0001-7869-9411], Redondo-López, Arturo [0000-0003-2037-5400], González Schain, Nahuel [0000-0003-2446-5825], Allona, Isabel [0000-0002-7012-2850], Wabnik, Krzysztof [0000-0001-7263-0560], Perales, Mariano [0000-0002-7351-8439], Alique, Daniel, Redondo-López, Arturo, González Schain, Nahuel, Allona, Isabel, Wabnik, Krzysztof, and Perales, Mariano

Abstract

Poplar trees use photoperiod as a precise seasonal indicator, synchronizing plant phenology with the environment. Daylength cue determines FLOWERING LOCUS T 2 (FT2) daily expression, crucial for shoot apex development and establishment of the annual growing period. However, limited evidence exists for the molecular factors controlling FT2 transcription and the conservation with the photoperiodic control of Arabidopsis flowering. We demonstrate that FT2 expression mediates growth cessation response quantitatively, and we provide a minimal data-driven model linking core clock genes to FT2 daily levels. GIGANTEA (GI) emerges as a critical inducer of the FT2 activation window, time-bound by TIMING OF CAB EXPRESSION (TOC1) and LATE ELONGATED HYPOCOTYL (LHY2) repressions. CRISPR/Cas9 loss-of-function lines validate these roles, identifying TOC1 as a long-sought FT2 repressor. Additionally, model simulations predict that FT2 downregulation upon daylength shortening results from a progressive narrowing of this activation window, driven by the phase shift observed in the preceding clock genes. This circadian-mediated mechanism enables poplar to exploit FT2 levels as an accurate daylength-meter.

Published
2024

2. Two orthogonal differentiation gradients locally coordinate fruit morphogenesis

Author

Fonds de Recherche du Québec, Natural Sciences and Engineering Research Council of Canada, Comunidad de Madrid, Agencia Estatal de Investigación (España), Gómez-Felipe, Andrea [0000-0002-5647-0496], Branchini, Elvis [0000-0001-5826-7232], Wang, Binghan [0000-0003-0739-6741], Marconi, Marco [0000-0002-3457-1384], Stan, Teodora [0000-0001-6781-447X], Burkiewicz, Jérôme [0000-0001-6968-3506], de Folter, Stefan [0000-0003-4363-7274], Routier-Kierzkowska, Anne-Lise [0000-0003-0383-0811], Wabnik, Krzysztof [0000-0001-7263-0560], Kierzkowski, Daniel [0000-0002-1947-8691], Gómez-Felipe, Andrea, Branchini, Elvis, Wang, Binghan, Marconi, Marco, Bertrand-Rakusová, Hana, Stan, Teodora, Burkiewicz, Jérôme, de Folter, Stefan, Routier-Kierzkowska, Anne-Lise, Wabnik, Krzysztof, Kierzkowski, Daniel, Fonds de Recherche du Québec, Natural Sciences and Engineering Research Council of Canada, Comunidad de Madrid, Agencia Estatal de Investigación (España), Gómez-Felipe, Andrea [0000-0002-5647-0496], Branchini, Elvis [0000-0001-5826-7232], Wang, Binghan [0000-0003-0739-6741], Marconi, Marco [0000-0002-3457-1384], Stan, Teodora [0000-0001-6781-447X], Burkiewicz, Jérôme [0000-0001-6968-3506], de Folter, Stefan [0000-0003-4363-7274], Routier-Kierzkowska, Anne-Lise [0000-0003-0383-0811], Wabnik, Krzysztof [0000-0001-7263-0560], Kierzkowski, Daniel [0000-0002-1947-8691], Gómez-Felipe, Andrea, Branchini, Elvis, Wang, Binghan, Marconi, Marco, Bertrand-Rakusová, Hana, Stan, Teodora, Burkiewicz, Jérôme, de Folter, Stefan, Routier-Kierzkowska, Anne-Lise, Wabnik, Krzysztof, and Kierzkowski, Daniel

Abstract

Morphogenesis requires the coordination of cellular behaviors along developmental axes. In plants, gradients of growth and differentiation are typically established along a single longitudinal primordium axis to control global organ shape. Yet, it remains unclear how these gradients are locally adjusted to regulate the formation of complex organs that consist of diverse tissue types. Here we combine quantitative live imaging at cellular resolution with genetics, and chemical treatments to understand the formation of Arabidopsis thaliana female reproductive organ (gynoecium). We show that, contrary to other aerial organs, gynoecium shape is determined by two orthogonal, time-shifted differentiation gradients. An early mediolateral gradient controls valve morphogenesis while a late, longitudinal gradient regulates style differentiation. Local, tissue-dependent action of these gradients serves to fine-tune the common developmental program governing organ morphogenesis to ensure the specialized function of the gynoecium.

Published
2024

4. Two orthogonal differentiation gradients locally coordinate fruit morphogenesis.

Author

Gómez-Felipe, Andrea, Branchini, Elvis, Wang, Binghan, Marconi, Marco, Bertrand-Rakusová, Hana, Stan, Teodora, Burkiewicz, Jérôme, de Folter, Stefan, Routier-Kierzkowska, Anne-Lise, Wabnik, Krzysztof, and Kierzkowski, Daniel

Subjects
FEMALE reproductive organs, MORPHOGENESIS, CELL imaging, DEVELOPMENTAL programs, FRUIT development
Abstract

Morphogenesis requires the coordination of cellular behaviors along developmental axes. In plants, gradients of growth and differentiation are typically established along a single longitudinal primordium axis to control global organ shape. Yet, it remains unclear how these gradients are locally adjusted to regulate the formation of complex organs that consist of diverse tissue types. Here we combine quantitative live imaging at cellular resolution with genetics, and chemical treatments to understand the formation of Arabidopsis thaliana female reproductive organ (gynoecium). We show that, contrary to other aerial organs, gynoecium shape is determined by two orthogonal, time-shifted differentiation gradients. An early mediolateral gradient controls valve morphogenesis while a late, longitudinal gradient regulates style differentiation. Local, tissue-dependent action of these gradients serves to fine-tune the common developmental program governing organ morphogenesis to ensure the specialized function of the gynoecium. The coordination of cellular behaviors is essential for proper organogenesis. Here the authors show that fruit development in Arabidopsis is governed by time-shifted differentiation gradients that act locally along two perpendicular organ axes. [ABSTRACT FROM AUTHOR]

Published
2024
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