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Abstract The ecosystem system services framework has potential to help clarify wildlife management challenges at the single species scale, but existing methods struggle to capture the complex values and tradeoffs at play in human–wildlife interactions. We worked with community scientists to gather and use stories (n = 150) as a source of ecosystem services data about living alongside eastern coyotes (Canis latrans var) in rural Vermont, United States. Our a priori ecosystem service assessment showed that human–coyote interactions can have simultaneous positive and negative human well‐being impacts at both the sample scale and for individual interviewees. Our research identified emergent themes that lent insight into how interviewees justified different kinds of relationships with coyotes. We applied a tradeoffs lens to evaluate three policy options based on their potential to mitigate ecosystem disservices and maximize ecosystem services from human–coyote interactions. We found that ecosystem services assessment rooted in a dataset of stories revealed policy‐relevant understanding of value conflicts and alignments at the sample scale without overshadowing the nuances of individual interviewees' experiences.

Background: When subject to stress conditions such as nutrient limitation microalgae accumulate triacylglycerol (TAG). Fatty acid, a substrate for TAG synthesis is derived from de novo synthesis or by membrane remodeling. The model industrial alga Chlorellasorokiniana accumulates TAG and other storage compounds under nitrogen (N)-limited growth. Molecular mechanisms underlying these processes are still to be elucidated., Result: Previously we used transcriptomics to explore the regulation of TAG synthesis in C. sorokiniana. Surprisingly, our analysis showed that the expression of several key genes encoding enzymes involved in plastidic fatty acid synthesis are significantly repressed. Metabolic labeling with radiolabeled acetate showed that de novo fatty acid synthesis is indeed downregulated under N-limitation. Likewise, inhibition of the Target of Rapamycin kinase (TOR), a key regulator of metabolism and growth, decreased fatty acid synthesis. We compared the changes in proteins and phosphoprotein abundance using a proteomics and phosphoproteomics approach in C. sorokiniana cells under N-limitation or TOR inhibition and found extensive overlap between the N-limited and TOR-inhibited conditions. We also identified changes in the phosphorylation status of TOR complex proteins, TOR-kinase, and RAPTOR, under N-limitation. This indicates that TOR signaling is altered in a nitrogen-dependent manner. We find that TOR-mediated metabolic remodeling of fatty acid synthesis under N-limitation is conserved in the chlorophyte algae Chlorella sorokiniana and Chlamydomonas reinhardtii., Conclusion: Our results indicate that under N-limitation there is significant metabolic remodeling, including fatty acid synthesis, mediated by TOR signaling. This process is conserved across chlorophyte algae. Using proteomic and phosphoproteomic analysis, we show that N-limitation affects TOR signaling and this in-turn affects the metabolic status of the cells. This study presents a link between N-limitation, TOR signaling and fatty acid synthesis in green-lineage., (© 2024. The Author(s).)

Many microbes accumulate energy storage molecules such as triglycerides (TAG) and starch during nutrient limitation. In eukaryotic green algae grown under nitrogen-limiting conditions, triglyceride accumulation is coupled with chlorosis and growth arrest. In this study, we show that reactive oxygen species (ROS) actively accumulate during nitrogen limitation in the microalga Chlorella sorokiniana . Accumulation of ROS is mediated by the downregulation of genes encoding ROS-quenching enzymes, such as superoxide dismutases, catalase, peroxiredoxin, and glutathione peroxidase-like, and by the upregulation of enzymes involved in generating ROS, such as NADPH oxidase, xanthine oxidase, and amine oxidases. The expression of genes involved in ascorbate and glutathione metabolism is also affected under this condition. ROS accumulation contributes to the degradation of monogalactosyl diacylglycerol (MGDG) and thylakoid membrane remodeling, leading to chlorosis. Quenching ROS under nitrogen limitation reduces the degradation of MGDG and the accumulation of TAG. This work shows that ROS accumulation, membrane remodeling, and TAG accumulation under nitrogen limitation are intricately linked in the microalga C. sorokiniana., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Vijayan, Wase, Liu, Morse, Zhang and Riekhof.)

Successful management of invasive species often requires working across public and private landownerships. A prime example of an invasive species that commonly occurs on privately and publicly owned and managed lands is the wild pig (Sus scrofa). Because of the multitude of negative impacts associated with wild pigs, management must occur across both private and public lands to achieve widespread control and sustained success. However, managing wild pigs across property boundaries is challenging as we know very little about differing management practices and landowner perspectives. To address this knowledge gap, we sought to understand wild pig management efforts on privately owned lands, the perceived economic, ecological, and human health impact of wild pigs, and beliefs related to policy. Generally, stakeholders believe wild pigs have negative impacts on wildlife, the economy, and ecological and public health, however less than half of landowners participate in wild pig control. Furthermore, stakeholders believe that the responsibility of managing and paying for damages associated with wild pigs lies with individual landowners. Our findings suggest that increased efficacy of wild pig control and collaboration between private and public landowners is not only possible but also necessary if wild pig population control is to be regionally successful., (© 2024. The Author(s).)