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Elephantopus mollis Kunth H.B et Kunth is an herbal plant employed customarily for the treatment of numerous maladies, notably cancers. Here in this research, we studied the effects of E. mollis (EM) petroleum ether extract (EM-PE) on the highly aggressive breast cancer cell line MDA-MB-231. The result from phytochemical analysis demonstrated the presence of tannins and saponins in EM-PE, of which, saponins made up more than 50% of the extract's mass. Cytotoxicity results, which were obtained from MTT assay and microscopic observation, suggested the potential of EM-PE to inhibit the growth of MDA-MB-231 cells with low IC50 value (approximately 30 μg/mL) and remarkably high selectivity index (> 4.78). Further evaluation indicated that EM-PE inhibited MDA-MB-231 cells growth in a dose-dependent manner. Interestingly, we found that EM-PE induced senescence in MDA-MB-231 cells via the activation of senescence-associated β-galactosidase and the transcriptional upregulation of p21 (3.7 times) and p27 (1.4 times). In consistent with this effect, pre-treated cancer cells showed no proliferative recovery after EM-PE removal. In addition, EM-PE could dramatically hinder breast cancer cells invasion (as much as 15.07-fold), which was shown in Transwell invasion assay, together with the decreased transcription of the important metastatic-involved SNAIL1 gene. Overall, our study, for the first time, exhibits the anti-proliferation and anti-invasion effects of EM extract on highly metastasis breast cancer cell line MDA-MB-231. Hence, these findings contributed to the knowledge of anti-cancer potential of this herbal plant. [ABSTRACT FROM AUTHOR]