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This article discusses the engineering of soybean plants with high levels of herbicide resistance using a Cas12-SF01-based cytosine base editor. Non-transgenic herbicide-tolerant soybean varieties are in demand due to the challenges posed by weeds in soybean cultivation. The study explores the use of amino acid substitution mutations in multiple ALS genes to produce soybeans with sufficient herbicide resistance. The researchers successfully generated herbicide-resistant soybean mutants and evaluated their resistance in greenhouse and field experiments. The results showed that the mutants exhibited strong herbicide resistance without affecting plant productivity. This study demonstrates the effectiveness of the base editor system for breeding herbicide-tolerant soybeans. [Extracted from the article]

A recent article in the Journal of Integrative Plant Biology discusses a simple method for transforming and editing genes in medicinal plants. The method, called Cut-Dip-Budding (CDB), bypasses the need for tissue culture and hairy root formation, making it more efficient and successful than traditional gene delivery methods. The researchers successfully used the CDB method to transform and edit genes in several medicinal plant species, including Pugongying, Dihuang, Danshen, and Yuanzhi. This breakthrough has the potential to greatly expand the number of medicinal plants that can be genetically modified and studied. [Extracted from the article]