1. Antioxidant and anti-cancer potentials of Ag green-synthesized and encapsulated olive leaves particles on HCT-116 cells.
- Author
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Mansour HMM, Shehata MG, Darwish AMG, Hafez EE, Samy MA, Abdelmotilib NM, and Abdo EM
- Subjects
- Humans, HCT116 Cells, Animals, Vero Cells, Chlorocebus aethiops, Antineoplastic Agents pharmacology, Antineoplastic Agents chemistry, Cell Survival drug effects, Particle Size, Olea chemistry, Silver chemistry, Silver pharmacology, Plant Leaves chemistry, Antioxidants pharmacology, Antioxidants chemistry, Metal Nanoparticles chemistry, Green Chemistry Technology, Plant Extracts pharmacology, Plant Extracts chemistry
- Abstract
Water extracts (OLE), whey protein encapsulated extracts (OLE/WPNs), and silver nanoparticles (OLE/Ag-NPs) were prepared from olive leaves of Manzenllie and Picual varieties. These preparations were characterized, and their antioxidant and biological activities on Vero and HCT-116 colorectal cells were assessed. The mechanism of action of the preparations was studied through tumor necrosis factor-α (TNF-α) and cytochrome C oxidase (Cox1) gene expression. OLE/Ag-NPs showed smaller particle sizes (14.23-15.53 nm) than OLE/WPNs (229.83-310.67 nm) and demonstrated lower aggregation due to their high Ƹ-potential of -24.86 to -27.90 mV. None of the preparations affected the viability of Vero cells (IC
50 = 192.19-421.01 μg/mL), but they showed cytotoxic effects on HCT-116 cells (IC50 = 50.76-196.54 μg/mL), particularly OLE/WPNs. Moreover, the preparations from the Picual variety (OLE, OLE/WPNs, and OLE/Ag-NPs) showed regulatory effects against colon cancer on treated HCT-116 cells by upregulating Cox1 expression and downregulating TNF-α expression. Consequently, OLE/WPNs and OLE/Ag-NPs could be promising for industrial applications with potential health benefits., Competing Interests: Declaration of competing interest The authors have no conflicts of interest to declare., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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