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The influence of barchan dune interaction upon unsteady flow separation and wake dynamics around the fixed-bed downstream barchan dune (DBD) model are experimentally investigated at a Reynolds number of 2640 based on the tomographic particle image velocimetry (PIV) system. The time-averaged statistics including the mean velocities, recirculation area, vortex spatial topology, Reynolds stress, and turbulent kinetic energy were used to characterize the flow field and large-scale anisotropy. It was found that arch-shaped vortex "chains" with strong spanwise coherence shedding from isolated barchan crestline populate the whole wake region, while elongated rod-shaped vortex structures with strong streamwise coherence induced by the up-downwash flow around the DBD were found to fill the whole measurement range, which is closely related to "sheltering" effect on the incoming flow acting at DBD due to the presence of upstream barchan dune (UBD). Additionally, in order to study the complex dynamic features of these predominated vortex structure transformations, time-resolved planar particle image velocimetry was applied. This technique allows for providing complementary insights into the temporal behavior of the unsteady coherent flow structures populating the wake field in different experimental configurations. It was found that the basic unsteady flapping motion, vortex roll-up, and complex vortex interactions including vortex pairing, merging, and breaking up can all be analyzed by dividing into certain scales with ease in a combination wavelet and Lagrangian framework. [ABSTRACT FROM AUTHOR]