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Resumo As empresas-plataforma voltadas ao trabalho de cuidado expandiram-se velozmente nos últimos anos no Brasil, em especial após a recente crise sanitária. Tal expansão trouxe para a ordem do dia o tema deste artigo, a saber, os possíveis efeitos da pandemia de Covid-19 sobre a digitalização da intermediação do trabalho nos cuidados, um segmento para o qual a literatura brasileira sobre plataformização do trabalho tem estado pouco atenta. Para acompanhar o ritmo desse crescimento, várias estratégias foram adotadas diante do desafio da falta de dados desagregados para o setor. Assim, mapeamos os aplicativos e plataformas de cuidado, construindo um banco de aplicativos a partir de dados da principal loja de apps no país; bem assim, analisamos os sites, os Termos de Serviços e Condições de Uso de três plataformas de cuidado direto e indireto. Conduzimos, ainda, entrevistas com trabalhadoras nessas plataformas e analisamos suas denúncias postadas no site Reclame Aqui. Concluímos que a governança das plataformas afetou o cotidiano das trabalhadoras sob diferentes formas e com efeitos variados conforme cada plataforma. Entre eles, destacamos o diálogo com os clientes, o controle via sistema de notas prescritivas e punitivas, a inclusão de tarefas que iam além da atividade contratada, bem como a sobreposição de diferentes formas de cuidado. Ter documentado a intensificação do trabalho de cuidado e a capacidade manifesta pela empresa-plataforma de ir além do mero pareamento entre demandantes e ofertantes, passando a intervir no modo como o próprio trabalho se exerce, é a contribuição desta pesquisa para uma análise das formas assumidas pelo amplo e recente processo de digitalização do trabalho de cuidado no Brasil.

Abstract Pyruvate dehydrogenase (PDH) catalyses the irreversible decarboxylation of pyruvate to acetyl‐CoA, which feeds the tricarboxylic acid cycle. We investigated how the loss of PDH affects metabolism in Pseudomonas putida. PDH inactivation resulted in a strain unable to utilize compounds whose assimilation converges at pyruvate, including sugars and several amino acids, whereas compounds that generate acetyl‐CoA supported growth. PDH inactivation also resulted in the loss of carbon catabolite repression (CCR), which inhibits the assimilation of non‐preferred compounds in the presence of other preferred compounds. Pseudomonas putida can degrade many aromatic compounds, most of which produce acetyl‐CoA, making it useful for biotransformation and bioremediation. However, the genes involved in these metabolic pathways are often inhibited by CCR when glucose or amino acids are also present. Our results demonstrate that the PDH‐null strain can efficiently degrade aromatic compounds even in the presence of other preferred substrates, which the wild‐type strain does inefficiently, or not at all. As the loss of PDH limits the assimilation of many sugars and amino acids and relieves the CCR, the PDH‐null strain could be useful in biotransformation or bioremediation processes that require growth with mixtures of preferred substrates and aromatic compounds.

Introduction: The Paget-Schroetter Syndrome is one in which the compression of the axillary and subclavian veins generates thrombosis, leading to blood stasis in the venous system of the upper limb and its respective complications. First-line treatment focuses on thrombolysis and anticoagulation of patients. Case presentation: A 23-year-old female patient attended the emergency department due to edema and erythema of the right thoracic extremity for 1 day, as well as pain in the axillary region when moving the arm and infraclavicular pain on palpation, and paresthesia in the fingers, that started 2 hours before consultation. Conclusion: However, the debate continues as to whether the resection of the first rib as a decompression method should be applied to all patients or whether, depending on the characteristics of each patient, it is possible to follow other regimes to avoid the recurrence of symptoms and even subsequent thrombosis episodes.

Abstract Metabolically versatile bacteria exhibit a global regulatory response known as carbon catabolite repression (CCR), which prioritizes some carbon sources over others when all are present in sufficient amounts. This optimizes growth by distributing metabolite fluxes, but can restrict yields in biotechnological applications. The molecular mechanisms and preferred substrates for CCR vary between bacterial groups. Escherichia coli prioritizes glucose whereas Pseudomonas sp. prefer certain organic acids or amino acids. A significant issue in understanding (and potentially bypassing) CCR is the lack of information about the signals that trigger this regulatory response. In E. coli, several key compounds act as flux sensors, governing the flow of metabolites through catabolic pathways and preventing imbalances. These flux sensors can also modulate the CCR response. It has been suggested that the order of substrate preference is determined by carbon uptake flux rather than substrate identity. For Pseudomonas, much less information is available, as the signals that induce CCR are poorly understood. This article briefly discusses the available evidence on the signals that trigger CCR and the questions that remain to be answered in Pseudomonas.