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Despite the widely recognized role of pollinators in ecosystem services, we currently have a poor understanding of the contribution of Natural Protected Areas neighboring agricultural landscapes to crop pollinator diversity and plant-pollinator interactions. Here, we conducted monthly surveys over a period of one year to study the diversity of insect visitors in dominant fruit crops-avocado, plum, apple, and blackberry-and used pollen DNA metabarcoding to characterize the community of plant sources in and around low-intensive farmland bordered by protected montane forest in Costa Rica. We found that crops and native plants had distinct communities of flower visitors, suggesting the presence of fine-scale habitat differences. DNA metabarcoding coupled with a custom-built reference database, enabled us to identify plant sources among pollen samples with high taxonomic resolution (species or genus level). We found that insect visitors carried pollen from a large diversity of plant taxa, including species native to the montane forests and highland páramos of Costa Rica. The diversity and composition of plant sources were variable across fruit crops and insect groups. Wildflower visitors such as bumblebees and syrphid flies, use a diverse range of plant taxa at similar levels to managed honeybees. This indicates the potential contribution of a diverse community of insect visitors to the pollination services of fruit crops and native flora. Overall, our study suggests that low-intensive farming practices that promote the presence of common ruderals combined with nearby protected forests contribute to maintaining diverse insect communities that provide crucial pollination services., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2025 Montero, Gamboa-Barrantes, Rojas-Malavasi, Cristóbal-Perez, Barrantes, Cascante-Marín, Hanson, Zumbado, Madrigal-Brenes, Martén-Rodríguez, Quesada and Fuchs.)

Background: Delabelling pathways offer confirmatory diagnosis and can prevent unnecessary second-line therapies or drug desensitization procedures after chemotherapeutic hypersensitivity reactions (CHT-HSRs). However, these pathways rely on risky in vivo tests. Data on whether in vitro tests could be helpful are scarce. We assessed the role of basophil activation test (BAT) in the diagnosis of HSRs to platin salts (PSs) and taxanes (TXs) in a well-defined population featuring varied endophenotypes and severities of HSRs., Methods: We conducted a 3-year-long multicentric, prospective study with 121 suspected-immediate CHT-HSR patients. The allergy workup included clinical history (initial reaction based on Type I, cytokine release syndrome, and mixed phenotype's symptoms and if unable to fit in any of these, as "indeterminate"), skin testing (ST), and drug provocation testing (DPT), provided risk assessment was favorable. Final diagnosis classified patients as "hypersensitive," "non-hypersensitive," or "inconclusive." We performed BAT using CD63 and CD203c as activation markers in patients and controls. Patients underwent DPT regardless of BAT results to prevent bias., Results: ST positivity significantly correlated with skin involvement, Type I phenotype, cancer recurrence, and lifetime exposures before reactions. DPTs were negative in all indeterminate phenotype patients (p = .02) and those considered low-risk, whereas they were negative in 62% moderate-risk patients. 55% were confirmed as hypersensitive (mainly Type I reactions, p < .0001), 24% as non-hypersensitive (mainly TXs and indeterminate phenotypes), and 21% as inconclusive. BAT showed 79% sensitivity in Type I IgE-mediated reactions to PSs with a high correlation to ST., Conclusions: BAT is a promising tool for delabelling and endotyping CHT-HSRs, especially Type I reactions to PSs, possibly identifying patients at risk of positive DPT. ST seems useful in confirming CHT-HSRs, especially PS-induced reactions, and DPT remains the gold standard, being essential even in moderate-risk patients., (© 2024 The Author(s). Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd.)