1. Differential detection of megakaryocytic and erythroid DNA in plasma in hematological disorders
- Author
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W. K. Jacky Lam, Wanxia Gai, Jinyue Bai, Tommy H. C. Tam, Wai Fung Cheung, Lu Ji, Irene O. L. Tse, Amy F. C. Tsang, Maggie Z. J. Li, Peiyong Jiang, Man Fai Law, Raymond S. M. Wong, K. C. Allen Chan, and Y. M. Dennis Lo
- Subjects
Medicine ,Genetics ,QH426-470 - Abstract
Abstract The tissues of origin of plasma DNA can be revealed by methylation patterns. However, the relative DNA contributions from megakaryocytes and erythroblasts into plasma appeared inconsistent among studies. To shed light into this phenomenon, we developed droplet digital PCR (ddPCR) assays for the differential detection of contributions from these cell types in plasma based on megakaryocyte-specific and erythroblast-specific methylation markers. Megakaryocytic DNA and erythroid DNA contributed a median of 44.2% and 6.2% in healthy individuals, respectively. Patients with idiopathic thrombocytopenic purpura had a significantly higher proportion of megakaryocytic DNA in plasma compared to healthy controls (median: 59.9% versus 44.2%; P = 0.03). Similarly, patients with β-thalassemia were shown to have higher proportions of plasma erythroid DNA compared to healthy controls (median: 50.9% versus 6.2%) (P
- Published
- 2024
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