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The objective of this study was to evaluate the impact of early progesterone removal on pregnancy rates to fixed-time artificial insemination (FTAI) in presynchronized beef cows. Postpartum beef cows (n = 882) were randomly assigned to 1 of 2 treatments: 1) 7&7 Synch : cows received a controlled internal drug release insert (CIDR) and a 25-mg injection of prostaglandin F 2α on day 0, 100 μg of GnRH on day 7, a second injection of prostaglandin F 2α (PG2) at CIDR removal on day 14, and a second injection of GnRH at FTAI 60–66 h after PG2 (day 17); 2) 7&6 Synch : cows received the same treatment as 7&7 Synch; however, CIDR removal occurred in conjunction with PG2 on day 13, while FTAI remained at 60–66 h after CIDR removal (day 16). Ovarian ultrasonography was performed to determine follicle diameter at PG2 and FTAI in a subset of cows (n = 40). Cows exposed to the 7&7 Synch tended to have larger follicle diameter at PG2 compared with 7&6 Synch cows (P = 0.09); however, there were no differences in follicle diameter at FTAI. No differences were determined between treatments for the expression of estrus (7&7 Synch: 61.6 ± 5.30; 7&6 Synch: 54.1 ± 5.45; P = 0.31) or pregnancy rates to FTAI (7&7 Synch: 60.8 ± 3.83; 7&6 Synch: 57.0 ± 3.84; P = 0.42). In conclusion, early removal of progesterone did not impact pregnancy rates in presynchronized beef cows. • Cows with greater days postpartum had greater expression of estrus. • Presynchronized cows that expressed estrus behavior had greater pregnancy rates. • 7&6 Synch cows tended to have smaller follicles at progesterone withdrawal. • 7&7 Synch and 7&6 Synch treatments resulted in similar expression of estrus. • 7&7 Synch and 7&6 Synch treatments resulted in similar pregnancy rates. [ABSTRACT FROM AUTHOR]

The aims of this study were to: 1) evaluate the impact of intravaginal progesterone (P4) inserts containing different amounts of P4 on pregnancy rates of predominantly Bos taurus beef cows exposed to fixed-time artificial insemination (FTAI) using estradiol and P4-based synchronization protocols, and 2) evaluate the impact of delayed luteolysis on the fertility of cows receiving P4 insert with less P4. Cows (n = 1744) were randomly assigned to 1 of 3 treatments: 1) 2.0 mg of estradiol benzoate together with an intravaginal P4-releasing insert containing 1.9 g of P4 (CIDR) on Day 0; 12.5 mg of dinoprost tromethamine on Day 7; 300-IU of equine chorionic gonadotropin together with 0.6 mg of estradiol cypionate and CIDR withdrawal on Day 9 (CIDR-PG7); 2) cows were exposed to the same treatment as CIDR-PG7, however a different intravaginal insert containing 0.5 g of P4 (DIB 0.5) was utilized (DIB-PG7); and 3) cows were exposed to the same synchronization treatment as DIB-PG7, however a dinoprost tromethamine injection was administered on Day 9 instead of Day 7 (DIB-PG9). All cows received a fixed-time artificial insemination (FTAI) 48 h after P4 inserts CIDR removal (Day 11). Circulating concentrations of P4 on Day 9 were greater (P < 0.01) in cows receiving CIDR-PG7 than in cows receiving DIB-PG7 or DIB-PG9. Dominant follicle diameter on Day 9 was greater (P < 0.01) in DIB-PG7 compared with CIDR-PG7 or DIB-PG9, but not different (P > 0.10) between CIDR-PG7 and DIB-PG9 treatments. Follicle diameter on Day 11 did not differ between CIDR-PG7 and DIB-PG7 (P > 0.10), which were greater compared with DIB-PG9 (P < 0.01). Dominant follicle growth between Days 9 and 11 was greater (P < 0.01) in cows receiving CIDR-PG7 than in cows receiving DIB-PG7 and DIB-PG9, which were not different (P = 0.18) among them. Pregnancy rates on experimental Days 41 and 131 were greater (P < 0.01) in cows receiving CIDR-PG7 (59.4 %; 55.5 %) compared with DIB-PG7 (40.1 %; 34.4 %) or DIB-PG9 (45.2 %; 41.4 %) cows. Compared with cows receiving DIB-PG7, cows receiving DIB-PG9 treatment tended (P = 0.08) to have a greater pregnancy rate on experimental Day 41 and 131 (P = 0.06). In summary, intravaginal P4 inserts with only 0.5 g of P4 resulted in decreased circulating concentrations of P4 and decreased pregnancy rates. Delaying induction of luteolysis only partially mitigated this negative outcome., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)