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Despite undeniable advances in research on language learning strategies in the last several decades, empirical investigations of actions and thoughts that learners engage in to better understand and use grammar structures in different contexts, or grammar learning strategies (GLS), remain scarce. Moreover, there is a paucity of studies examining the effects of pedagogical interventions in this area, both with respect to the use of different types of GLS and the mastery of grammar structures in terms of explicit and implicit (automatized) knowledge. There is also no empirical evidence concerning the mediating role of individual difference (ID) factors in this respect. The paper outlines a study currently in progress that seeks to address all of these gaps. The first part is devoted to a brief overview of key issues related to the definition and classification of GLS as well as a synthesis of available research in this area. This is followed by a description of an intervention-based research project which explores the effects of strategies-based instruction (SBI) targeting GLS by English majors in Poland, also taking into account the moderating impact of selected ID variables. In particular, the methodology of the study is presented and the envisaged contributions of the intervention are considered.

The present research used a longitudinal framework to test a model of willingness to communicate (L2WTC), informal digital learning of English (IDLE), and foreign language boredom (FLB) in learning English as a foreign language among 325 Iranian university students attending a course in general English for 4 months via a latent change score mediation (LCSM) model. Overall, model indices showed an acceptable model fit for the LCSM model. There was a significant and positive constant growth in IDLE and L2WTC, a negative constant growth in FLB across four measurements, while the proportional change was significant and negative for all three variables. The significant interindividual and intraindividual differences in patterns of growth across time were found for the three variables. Also, moderate and positive cross-system associations were revealed between IDLE and L2WTC across four measurements. Moreover, moderate and negative cross-system associations were revealed between FLB and L2WTC as well as between IDLE and L2WTC. Furthermore, changes in FLB mediated subsequent associations between changes in IDLE and L2WTC. The results highlight the pivotal role of variations in FLB as a mediator for the longitudinal link between IDLE and L2WTC.

KAI2 receptors, classified as plant α/β hydrolase enzymes, are capable of perceiving smoke-derived butenolide signals and endogenous yet unidentified KAI2-ligands (KLs). While the number of functional KAI2 receptors varies among land plant species, rice has only one KAI2 gene. Rice, a significant crop and representative of grasses, relies on KAI2-mediated Arbuscular mycorrhiza (AM) symbioses to flourish in traditionally arid and nutrient-poor environments. This study presents the first crystal structure of an active rice (Oryza sativa, Os) KAI2 hydrolase receptor. Our structural and biochemical analyses uncover grass-unique pocket residues influencing ligand sensitivity and hydrolytic activity. Through structure-guided analysis, we identify a specific residue whose mutation enables the increase or decrease of ligand perception, catalytic activity, and signal transduction. Furthermore, we investigate OsKAI2-mediated signaling by examining its ability to form a complex with its binding partner, the F-box protein DWARF3 (D3) ubiquitin ligase and subsequent degradation of the target substrate OsSMAX1, demonstrating the significant role of hydrophobic interactions in the OsKAI2-D3 interface. This study provides new insights into the diverse and pivotal roles of the OsKAI2 signaling pathway in the plant kingdom, particularly in grasses.

The size of the human head is highly heritable, but genetic drivers of its variation within the general population remain unmapped. We perform a genome-wide association study on head size (N = 80,890) and identify 67 genetic loci, of which 50 are novel. Neuroimaging studies show that 17 variants affect specific brain areas, but most have widespread effects. Gene set enrichment is observed for various cancers and the p53, Wnt, and ErbB signaling pathways. Genes harboring lead variants are enriched for macrocephaly syndrome genes (37-fold) and high-fidelity cancer genes (9-fold), which is not seen for human height variants. Head size variants are also near genes preferentially expressed in intermediate progenitor cells, neural cells linked to evolutionary brain expansion. Our results indicate that genes regulating early brain and cranial growth incline to neoplasia later in life, irrespective of height. This warrants investigation of clinical implications of the link between head size and cancer.

Abstract Against conventional wisdom, corrugated grain boundaries in polycrystalline graphene, grown on Pt(111) surfaces, are shown to exhibit negative friction coefficients and non-monotonic velocity dependence. Using combined experimental, simulation, and modeling efforts, the underlying energy dissipation mechanism is found to be dominated by dynamic buckling of grain boundary dislocation protrusions. The revealed mechanism is expected to appear in a wide range of polycrystalline two-dimensional material interfaces, thus supporting the design of large-scale dry superlubric contacts.

As the global population continues growing, the demand for textiles also increases, putting pressure on cotton manufacturers to produce more natural fiber from this already undersupplied resource. Synthetic fibers such as polyester (PET) can be manufactured quickly and cheaply, but these petroleum-based products are detrimental to the environment. With increased efforts to encourage transparency and create a more circular textile economy, other natural alternatives must be considered. This article discusses the existing condition and future possibilities for man-made cellulosic fibers (MMCFs), with an emphasis on using non-woody alternative feedstocks as a starting material. This work focuses on conversion technology suitable for producing textile-grade fibers from non-wood-based dissolving pulp, which may be different in nature from its woody counterpart and therefore behave differently in spinning processes. Derivatization and dissolution methods are detailed, along with spinning techniques and parameters for these processes. Existing research related to the spinning of non-woody-based dissolving pulp is covered, along with suggestions for the most promising feedstock and technology combinations. In addition, an emerging method of conversion, in which textile fibers are spun from a hydrogel made of an undissolved nano/micro-fibrillated fiber suspension, is briefly discussed due to its unique potential. Methods and concepts compiled in this review relate to utilizing alternative feedstocks for future fibers while providing a better understanding of conventional and emerging fiber spinning processes for these fibers.

Abstract Background Nesfatin-1 is a neuropeptide that regulates the hypothalamic-pituitary-gonadal axis and may play a role in uterus function. It is co-expressed with other peptides, such as phoenixin, which can influence sex hormone secretion. Our previous research has confirmed that phoenixin-14 is involved in the development of cystic endometrial hyperplasia (CEH) and pyometra in dogs. Therefore, based on the similarities and interactions between these neuropeptides, we hypothesized that nesfatin-1 might also regulate the reproductive system in dogs. This study aimed to determine the expression of nesfatin-1 and its interaction with phoenixin-14 in dogs with CEH or pyometra compared to healthy females, and concerning animals’ body condition score (BCS 4–5/9 vs. BCS > 5/9). Results The analysis of nesfatin-1 in the uterus of bitches consisted of qPCR, western blot and immunofluorescence assays, and ELISAs. The results showed significantly higher nesfatin-1 encoding gene, nucleobindin-2 mRNA (Nucb2) and nesfatin-1 protein expression in overweight females and those suffering from CEH or pyometra compared to healthy animals. The immunoreactivity of nesfatin-1 was elevated in the uteri of bitches with higher BCS > 5/9. Moreover, nesfatin-1 blood concentrations increased in all examined overweight bitches. In the case of phoenixin signals, we found opposite results, regardless of the female body condition score. Conclusion The etiology of CEH and pyometra are not fully known, although we have expanded the level of knowledge with respect to the possible interaction of nesfatin-1 and phoenixin in female dogs’ uteri. They interact oppositely. With increasing female body weight, the expression of nesfatin-1 in the uterus and its peripheral blood concentration increased. However, for female dogs affected by CEH and pyometra, a decreased level of phoenixin-14, irrespective of their body condition score is characteristic. This knowledge could be crucial in the development of biomarkers for these conditions, which may lead to earlier recognition.

Abstract Mitochondria are essential eukaryotic organelles that regulate a range of cellular processes, from metabolism to calcium homeostasis and programmed cell death. They serve as essential platforms for antiviral signaling proteins during the innate immune response to viral infections. Mitochondria are dynamic structures, undergoing frequent fusion and fission processes that regulate various aspects of mitochondrial biology, including innate immunity. Pathogens have evolved sophisticated mechanisms to manipulate mitochondrial morphology and function to facilitate their replication. In this review, we examine the emerging literature on how flaviviruses modulate mitochondrial processes.

Abstract Background Zinc nanoparticles (NPs) are characterized by high bioavailability, small size, and high absorbability. The purpose of this experiment was to determine the effect of Zn-NP feed supplementation on ruminal fermentation, microbiota, and histopathology in lambs. In vitro (24 h), short-term (STE, 28 d), and long-term (LTE, 70 d) experiments were performed. The lambs in STE were fed a basal diet (BD) composed of 350 g/d ground barley and 700 g/d meadow hay (Control), BD enriched with ZnO-NPs (80 mg Zn/kg of diet, ZnO-NPs), and BD enriched with Zn phosphate-based NPs (80 mg Zn/kg of diet, ZnP-NP). The in vitro gas production technique was used in incubated rumen fluid from STE. The lambs in LTE were fed BD (Control), BD enriched with ZnO-NPs (40 mg Zn/kg of diet, ZnO-NP40), BD enriched with ZnO-NPs (80 mg Zn/kg of diet, ZnO-NP80) and BD enriched with ZnO (80 mg Zn/kg of diet, ZnO-80). Results After 24 h of incubation, dry matter digestibility was higher for ZnO-NP and ZnP-NP substrates than the control in an in vitro experiment (P

Abstract Background Traditional genomic profiling and mutation analysis of single cells like Circulating Tumor Cells (CTCs) fails to capture post-translational and functional alterations of proteins, often leading to limited treatment efficacy. To overcome this gap, we developed a miniaturized ‘protein analysis on the single cell level’ workflow—baptized ZeptoCTC. It integrates established technologies for single-cell isolation with sensitive Reverse Phase Protein Array (RPPA) analysis, thus enabling the comprehensive assessment of multiple protein expression and activation in individual CTCs. Methods The ZeptoCTC workflow involves several critical steps. Firstly, individual cells are labeled and isolated. This is followed by cell lysis and the printing of true single cell lysate preparations onto a ZeptoChip using a modified micromanipulator, CellCelector™. The printed lysates then undergo fluorescence immunoassay RPPA protein detection using a ZeptoReader. Finally, signal quantification is carried out with Image J software, ensuring precise measurement of multiple protein levels. Results The efficacy of ZeptoCTC was demonstrated through various applications. Initially, it was used for measuring EpCAM protein expression, a standard marker for CTC detection, revealing higher levels in single MCF-7 over MDA-MB-231 tumor cells. Furthermore, in Capivasertib (Akt-inhibitor)-treated MCF-7 single cells, ZeptoCTC detected a 2-fold increase in the pAkt/Akt ratio compared to control cells, and confirmed co-performed bulk-cell western blot analysis results. Notably, when applied to individual CTCs from metastasized breast cancer patients, ZeptoCTC revealed significant differences in protein activation levels, particularly in measured pAkt and pErk levels, compared to patient-matched WBCs. Moreover, it successfully differentiated between CTCs from patients with different Akt1 genotypes, highlighting its potential to determine the activation status of druggable cancer driving proteins for individual and targeted treatment decision making. Conclusions The ZeptoCTC workflow represents a valuable tool in single cell cancer research, crucial for personalized medicine. It permits detailed analysis of key proteins and their activation status of targeted, cancer-driven signaling pathways in single cell samples, aiding in understanding tumor response, progression, and treatment efficacy beyond bulk analysis. The method significantly advances clinical investigations in cancer, improving treatment precision and effectiveness. The workflow will be applicable to protein analysis on other types of single cells like relevant in stem cell, neuropathology and hemopoietic cell research.

We use a wide range of models (time series, semi-structural and structural) to estimate, project and explain the evolution of the natural rate of interest (NRI) in Poland (PL) and in the euro area (EA). Our findings are as follows: (i) the NRIs declined significantly in both PL and the EA over the last two decades, (ii) our most recent short-term estimates point towards a mildly negative NRI in PL and appreciably more negative NRI in the EA, (iii) our most recent medium-term estimates point towards a mildly positive NRI in PL and a slightly negative NRI in the EA, (iv) the NRI in PL remained consistently above the NRI in the EA by an average of 2–3 pp, (v) the main drivers of the declining NRIs were demographics and the worldwide productivity slowdown, (vi) the main driver of the higher NRI in PL than in the EA was the productivity catch-up in PL, (vii) we expect the NRI in PL to significantly converge to the EA level by the mid-2030s.

Experimental tests and numerical analyses were carried out on short, thin-walled channel columns with modified cross-sectional shape. The columns were loaded with an axial compressive force applied at the center of gravity of the cross section. Tests were carried out on a universal testing machine, while numerical analyses were performed in ANSYS software. The purpose of the tests was to determine the values of critical forces for the compressed columns and to determine the values of maximum forces at which failure of the columns occurs. Critical forces were determined based on the strain-averaged method. Based on the study, it was found that the strength and resistance to loss of stability of columns is primarily affected by their stiffness, and therefore by the shape of the cross-sections. In addition, for short columns, it seems more important to determine the value of maximum forces than the values of critical forces.

The biodegradation kinetics of paper materials with various chemical additives was studied, focusing on their potential tunability. Paper materials with additives, including retention aid, hydrophobic agent, and wet and dry strength agents, were explored in two forms: disintegrated fiber and paper form. Using the Gompertz equation, biodegradation kinetics were modeled to calculate the lag phase, initial biodegradation rate, and ultimate biodegradation extent. All paper materials showed higher biodegradation extents than microcrystalline cellulose (MCC) due to the highly biodegradable nature of hardwood bleached pulp. Disintegrated paper materials exhibited similar lag phase values and ultimate biodegradation to MCC regardless of treatment, while punched paper materials showed noticeable differences, suggesting that fiber disintegration plays a critical role in initiating biodegradation. Hydrophobic and wet strength treatments, such as alkyl ketone dimer (AKD) and wet-strength agents (PAE), respectively, significantly increased the lag phase, but their ultimate biodegradation extent remained intact. These findings highlight that the biodegradability of paper materials can be preserved even after chemical treatments, underscoring their environmentally friendly potential.

Abstract Depressive symptoms are highly prevalent and heterogeneous in women. Different brain structures might be associated with depressive symptoms and body composition in women with obesity/overweight and normal-/underweight, although the data is limited. The analysis included 265 women from Bialystok PLUS population study, untreated with antidepressive or antipsychotic medications. The subjects underwent brain magnetic resonance imaging and body composition analysis. Beck Depression Inventory (BDI) score was inversely associated with nucleus accumbens volume (β = −0.217, p = 0.008) in women with BMI ≥ 25 kg/m2, but with insula volume (β = −0.147, p = 0.027) in women with BMI

In this study we used a factor of curves model (FCM) to examine the co-development of the subdomains of boredom in practical English language classes in an online setting (BPELC) over time in four phases of an online L2 course to explore the covariance of the initial level and slope of the sub-domains, as well as to check to what extent the variation in these subdomains can be accounted by the underpinning global factor of BPELC. Data were collected from 412 adult EFL learners on four temporal occasions via the "Boredom in Practical English Classes--Revised scale," created by Pawlak et al. (2020a). Analysis was conducted by Mplus in three stages. Findings revealed a statically significant decrease over time in both subdomains. Also, a negative covariance was uncovered between the initial level and the growth level of each subdomain. Moreover, the covariances between the initial and growth levels of both subdomains were moderately high. Finally, the variances of the initial and growth levels of each of the subdomains were accounted for to a great extent by the underpinning global factor of BPELC. The results are interpreted in view of the underlying theories of boredom.

This paper describes the self-heating effects resulting from mechanical deformation in the additively manufactured aluminium alloy AlSi7Mg0.6. The material’s self-heating effect results from irreversible changes in the material’s microstructure that are directly coupled with the inelastic deformations. These processes are highly dissipative, which is reflected in the heat generation of the material. To describe such effects, a numerical framework that combines an elasto-viscoplastic Chaboche model with the Gurson Tvergaard Needleman damage approach is analysed and thermomechanically extended. This paper characterises the sample preparation, the experimental set-up, the development of the thermomechanical approach, and the material model. A user material subroutine applies the complete material model for the finite element software Abaqus 2022. To validate the material model and the parameters, a complex tensile test is performed. In order to check the finite element model, the energy transformation ratio is included in the evaluation. The numerical analyses of the mechanical stress evolution and the self-heating behaviour demonstrate good agreement with the experimental test. In addition, the calculation shows the expected behaviour of the void volume fraction that rises from the initial value of 0.0373% to a higher value under a complex mechanical load.

Objectives The aim of this work is to initiate or revive a scientific discussion on the impact of professional life on the parameters of human lifespan. Material and Methods Presented analysis is based on 8578 Polish elite or well-known person who died in 2001–2021. Results The results of the conducted analysis indicate that in the case of men the highest values of the median age at death were characteristic of freelancers (median [Me] ± quartile deviation [QD] 85.5±8.5 years), followed by scientists and academic teachers of the biological and medical specialty (Me±QD 84.0±7.5 years) and officers of power structures (Me±QD 83.5±8.5 years). Subsequently, the highest value of the median age at death was recorded for social activists (Me±QD 83.0±9.5 years), clergy (Me±QD 82.0±7.5 years) and scientists and academic teachers of specialties other than biological and medical (Me±QD 82.0±8.0 years). Significantly, at the very end of this list are athletes (Me±QD 77.0±9.0 years). Nevertheless, the results of the analysis confirm that professional athletes are characterized by higher median age at death compared to the general population. Analysis made only within athletes group demonstrated that the parameters of lifespan of athletes of endurance disciplines (Me±QD 78.0±8.0 years) are the most favorable compared to athletes of other disciplines, in particular in compare to team sports athletes (Me±QD 75.0±10.0 years) or combat sports athletes (Me±QD 75.0±7.1 years). Conclusions What is new and innovative in this paper is comparing the lifespan characteristics of athletes in comparison to widely represented group of other professions with higher socio-economic status. Unexpectedly, the lifespan of athletes occurred to be lower than for fast all other analyzed occupational groups, except mainly of entertainment musicians. Finally, the results presented in this paper emphasize the need to analyze the lifespan characteristics of athletes in a broader scope than only in relation to the general population. Int J Occup Med Environ Health 2024;37(3):335–50

Abstract Patients on left ventricular assist devices (LVAD) are prone to excessive hemostasis disturbances due to permanent contact of artificial pump surfaces with blood components. We aimed to investigate if fibrin clot permeability is altered in patients on long-term continuous-flow LVAD therapy and if the clot permeability is associated with clinical characteristics and adverse events. We investigated 85 end-stage heart failure patients (90.6% men, age 48.6–63.8 years) scheduled for continuous flow long-term LVAD support according to current clinical indications. The patients were assessed periodically: prior to LVAD implantation (T1), 3–6 months (T2) after LVAD implantation, 6–12 months after (T3) and then every 6 months. We tested the first three blood samples (T1-T3) and the last available blood sample (T4), but no longer than 5 years after LVAD implantation. We assessed hemostasis parameters (Activated Partial Thromboplastin Time (APTT) Prothrombin Time, Activated Partial Thromboplastin Time, Fibrinogen, d-dimer, Antithrombin, Thrombin Time, Factor VIII, and von Willebrand Factor, aspirin-induced platelet inhibition, adenosine-diphosphate test) changes during the study period. Fibrin Clot Permeability was evaluated using a pressure system and Permeability Coefficient (Ks) was calculated. We observed a decrease in fibrin clot permeability (Ks) between T1, T2, T3 and T4 time periods; P 0.001, however cumulative measurements in patients on aspirin showed shortening of Ks in this group P = 0.0123. Major adverse cardiac and cerebrovascular events (MACCE) occurred in 36.5% patients, bleeding events in 25.9%, Net Adverse Clinical Events (NACE) in 62.4%; 31.7% patients died, and 17.6% underwent transplantation. The transplantation was considered as the endpoint. Discrepancies in Ks were observed between patients with MACCE, bleeding, and NACE, and patients without adverse events. Ks showed a constant trend towards normalization (P

Artykuł omawia rolę antropologii technologii jako dyscypliny badawczej zorientowanej na przyszłość. Analizuje wpływ technologii, zwłaszcza transhumanistycznych idei, na kondycję współczesnego człowieka oraz przyszłe wyobrażenia postbiologicznej istoty. Przywołane zostały teoretyczne ujęcia z zakresu cyberpsychologii, filozofii techniki i humaniki, które wspierać mogą antropologię w badaniach nad rozwojem technologicznym i jego konsekwencjami społecznymi, kulturowymi i etycznymi. Artykuł podkreśla potrzebę angażowania antropologii w szeroką debatę publiczną na temat przyszłości ludzkości, konfrontacji wyobrażeń z rzeczywistością naukową oraz współpracy z naukowcami, artystami i społeczeństwem w kontekście szybkich zmian technologicznych. Wyzwania związane z transhumanizmem, rozwojem sztucznej inteligencji i ekspansją technologii wymagają interdyscyplinarnej perspektywy badawczej oraz aktywnego uczestnictwa antropologów w kształtowaniu przyszłości społeczeństwa.

Abstract Phosphorus pentamers (cyclo-P5) are unstable in nature but can be synthesized at the Ag(111) surface. Unlike monolayer black phosphorous, little is known about their electronic properties when in contact with metal electrodes, although this is crucial for future applications. Here, we characterize the atomic structure of cyclo-P5 assembled on Ag(111) using atomic force microscopy with functionalized tips and density functional theory. Combining force and tunneling spectroscopy, we find that a strong charge transfer induces an inward dipole moment at the cyclo-P5/Ag interface as well as the formation of an interface state. We probe the image potential states by field-effect resonant tunneling and quantify the increase of the local change of work function of 0.46 eV at the cyclo-P5 assembly. Our experimental approach suggest that the cyclo-P5/Ag interface has the characteristic ingredients of a p-type semiconductor-metal Schottky junction with potential applications in field-effect transistors, diodes, or solar cells.

Abstract Giant kelp (Macrocystis pyrifera) forests are common along the California coast. Attached on the rocky bottom at depths of approximately 5–25 m, the kelp, when mature, spans the water column and develops dense, buoyant canopies that interact with waves and currents. We present two novel results based on observations of surface gravity waves in a kelp forest in Point Loma, California. First, we report short wave (1–3 s) attenuation in kelp, quantified by an exponential decay coefficient α∼O10−3m−1—comparable to the dampening effect of sea ice. Second, we identify seasonal and tidal changes in attenuation, peaking mid‐summer with maximum kelp cover, and during low tide when a greater proportion of the fronds are at the surface. Thus, the naturally occurring surface canopies of kelp forests can act as temporally varying, high‐frequency filters of wave energy.