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Background: COVID-19 has been troublesome for families due to its socio-economic changes worldwide. Therefore, the present study aimed to investigate the socio-economic burden of COVID-19 on children’s health in Iran.Methods: In the present cross-sectional study, the target population was 500 parents of children aged 5 to 18 years from 5 provinces of Iran in 2021. The samples were randomly selected from different geographical regions of Iran. The questionnaires on the socioeconomic burden of COVID-19, general health, child health, and place of residence landscape were employed to collect the data. For data analysis, t-test, structural equation modeling (SEM) were used in SPSS version 26 and AMOS version 26, respectively.Results: The results indicated that based on determination coefficients of socio-economic burden of COVID-19, the socioeconomic status of the parents and the settlement external perspective variable account for nearly 25% of the mental changes in parents. Furthermore, the combined effects of COVID-19’s socioeconomic burden and parents’ mental health accounted for 26% of the health changes observed in children. The socio-economic burden had a negative correlation with parents’ mental health (β=-0.383, P

Background Ariidae species play a significant role as fishing resources in the Amazon region. However, the family’s systematic classification is notably challenging, particularly regarding species delimitation within certain genera. This difficulty arises from pronounced morphological similarities among species, posing obstacles to accurate species recognition. Methods Following morphological identification, mitochondrial markers (COI and Cytb) were employed to assess the diversity of Ariidae species in the Amazon. Results Our sampling efforts yielded 12 species, representing 92% of the coastal Amazon region’s diversity. Morphological identification findings were largely corroborated by molecular data, particularly for species within the Sciades and Bagre genera. Nonetheless, despite morphological support, Cathorops agassizii and Cathorops spixii displayed minimal genetic divergence (0.010). Similarly, Notarius quadriscutis and Notarius phrygiatus formed a single clade with no genetic divergence, indicating mitochondrial introgression. For the majority of taxa examined, both COI and Cytb demonstrated efficacy as DNA barcodes, with Cytb exhibiting greater polymorphism and resolution. Consequently, the molecular tools utilized proved highly effective for species discrimination and identification.

The Chilean meagre, Cilus gilberti, emerges as a novel species to South American aquaculture. Nevertheless, the successful establishment of its cultivation necessitates careful consideration of productive parameters and tolerance to abiotic stressors. To address these challenges, the identifications of biomarkers emerges as a promising approach. Indeed, leptin stands out as a potential biomarker, given its multifaceted role. This study focuses on characterizing leptin in C. gilberti (cgLep) and assessing its gene expression in juveniles exposed to different culture and feeding conditions. The results indicated that cgLep coding sequence yields a 137-amino acid prohormone, preserving high relevant cysteine residues for maintaining its structural integrity. Basal cgLep gene expression profiles reveal its expression mainly in the liver, followed by white muscle. Conversely, its receptor (cgLepR) exhibits higher levels in white muscle. Additionally, it was observed that juveniles in a postprandial state exhibited an upregulation of hepatic cgLep. Simultaneously, the cgLepR showed a significant increase at brain level during the preprandial stage. The exposure to acute hypoxia revealed a notable upregulation of cgLep after two hours of low oxygen availability and returned to normoxia baseline after six hours post-hypoxia. Notably, when subjected to a prolonged daily hypoxia regimen, no significant differences in leptin mRNA expression were observed. This suggests a dynamic adaptive response of the C. gilberti leptin system to hypoxia. Finally, juvenile Chilean meagre cultivated at 15 Kg/m3 showcase significant individual variability in hepatic leptin expression levels when compared to those cultured at 25 or 35 kg/m3. These observations highlight that leptin primarily expresses in the liver and its expression is notably influenced by fasting periods and chronic stress induced by low stock density. This variability in leptin expression could potentially impact food intake, emphasizing the importance of considering these factors in the design and implementation of aquaculture practices for Chilean meagre.

Abstract The Tambaqui is one of the most representative Amazon fish species, being highly exploited in fisheries, aquaculture and as a research model. Nonetheless, data about functional genome are still required to evaluate reproductive and nutrition parameters as well as resistance to pathogens. The of next-generation sequencing has allows assessing the transcriptional processes in non-model species by providing comprehensive gene collections to be used as a database in further genomic applications and increased performance of captive populations. In this study, we relied on RNAseq approach to generate the first transcriptome of the telencephalon from adult males and females of Colossoma macropomum, resulting in a reference dataset for future functional studies. We retrieved 896,238 transcripts, including the identification of 267,785 contigs and 203,790 genes. From this total, 91 transcripts were differentially expressed, being 63 and 28 of them positively regulated for females and males, respectively. The functional annotation resulted in a library of 40 candidate genes for females and 20 for males. The functional enrichment classes comprised reproductive processes (GO:0,048,609; GO:0,003,006; GO:0,044,703; GO:0,032,504; GO:0,019,953) being related to sex differentiation (e.g., SAFB) and immune response (e.g., SLC2A6, AHNAK, NLRC3, NLRP3 and IgC MHC I alpha3), thus indicating that the genes in the neurotranscriptome of Tambaqui participate in sex differentiation and homeostasis of captive specimens. These data are useful to design the selection of genes related to sex determination and animal welfare in raising systems of Tambaqui.

Abstract Background Standard cancer treatments show a lack of selectivity that has led to the search for new strategies against cancer. The selective elimination of cancer cells modulating the redox environment, known as “selective oxycution”, has emerged as a viable alternative. This research focuses on characterizing the unexplored Escallonia genus plant extracts and evaluating their potential effects on cancer’s redox balance, cytotoxicity, and activation of death pathways. Methods 36 plant extracts were obtained from 4 different species of the Escallonia genus (E. illinita C. Presl, E. rubra (Ruiz & Pav.) Pers., E. revoluta (Ruiz & Pav.) Pers., and E. pulverulenta (Ruiz & Pav.) Pers.), which were posteriorly analyzed by their phytoconstituents, antioxidant capacity, and GC-MS. Further, redox balance assays (antioxidant enzymes, oxidative damage, and transcription factors) and cytotoxic effects (SRB, ∆Ψmt, and caspases actives) of those plant extracts were analyzed on four cell lines (HEK-293T, MCF-7, HT-29, and PC-3). Results 36 plant extracts were obtained, and their phytoconstituents and antioxidant capacity were established. Further, only six extracts had EC50 values

Road network connectivity determines the accessibility of urban activities for pedestrians, while streetscape characteristics have an impact on route attractiveness. Methods used to measure the influence of connectivity and streetscape characteristics on walking behavior differ substantially, while trip purpose is a key factor. This paper explores the respective contributions of network connectivity and streetscape features to explain leisure walking and utilitarian trips on 740 street segments in Santarém (Portugal). The indicators cover the most commonly used factors in walkability indexes, such as density, diversity, design, and accessibility. The streetscape features measure imageability, enclosure, human scale, transparency, and complexity. The walking trip information was collected via survey. The results show that connectivity measures have a greater overall explanatory power for both trip motives. However, the findings highlight the need to consider a variety of design indicators to explain walking behavior due to the higher explanation power of the model with two types of indicators.

The aim of the present study was to investigate the communication/training strategies and technological tools used by fitness coaches from Brazilian football teams during home-based training according to age categories (professional and youth), competitive level (1st, 2nd and 3rd/4th divisions) and sex (male and female). An online questionnaire was applied to collect data. Eighty-two fitness coaches from sixty-seven male and female football teams (professional and youth teams) answered the questionnaire. Message apps were the main communication route between fitness coaches and players during home-based training. Online spreadsheets were frequently used to control the training. Regarding equipment provided to players for training, 70.7% of male professional players used some equipment. In contrast, most female professional and youth players (both sexes) did not receive any equipment, respectively (p<0.01). Male players who compete in the 1st and 2nd divisions received more equipment for training than those who compete in the 3rd and 4th divisions (p<0.01). The main physical fitness variables trained by all players during home-based training were muscle strength, strength-endurance, and aerobic capacity. Training load and training response were applied through series/repetitions/time and effort perception scales, respectively. In conclusion, the financial differences between professional and youth teams and between males and females and competitive levels can induce different home-based training conditions in Brazilian football teams during lockdown-like situations, especially related to equipment available for training targeted physical qualities. Thus, players should be monitored closely during lockdown-like situations and provided appropriate support to facilitate their training. [ABSTRACT FROM AUTHOR]

Abstract Background This study aims to investigate the prevalence and associated risk factors of inappropriate use of emergency services among young adults in Vitória, Brazil. Methods A cross-sectional study was conducted over 30 consecutive days in November and December 2019, involving systematic random sampling of young adults (aged 18–39) visiting the municipal emergency care unit. Data were collected through structured interviews, utilizing the Hospital Urgency Appropriateness Protocol (HUAP) to identify inappropriate use. Demographic characteristics, healthcare utilization patterns, and medical diagnoses were assessed. Poisson regression models were employed to explore associations between variables. Results Among the 631 young adults surveyed, 30.6% exhibited inappropriate use of the emergency care unit. Factors associated with higher rates of inappropriate use included not seeking previous care in other healthcare services; having specific medical diagnoses like diseases of the respiratory system (PR: 2.03), diseases of the skin (PR: 4.13), and diseases of the ear and mastoid (PR: 3.74). Conclusion The study underscores the significance of addressing inappropriate use of emergency services among young adults. Though the prevalence of inappropriate use was not significantly different from other age groups, the demographic characteristics and healthcare utilization patterns of young adults contribute to their unique challenges. To mitigate inappropriate use, efforts should focus on improving access to primary healthcare services, enhancing continuity of care, and raising awareness about appropriate healthcare-seeking behaviors among young adults. Ultimately, these interventions can foster a more effective and sustainable healthcare system that better serves the needs of the community.

A Atenção Primária à Saúde (APS) é considerada a porta de entrada preferencial para o cuidado no sistema de saúde; já os serviços de emergência são destinados para situações que exigem atendimento imediato. Este artigo buscou identificar motivações para o uso de pronto atendimentos por problemas de saúde não urgentes em usuários deste serviço. Trata-se de um estudo qualitativo baseado em 29 entrevistas semiestruturadas realizadas em 2019 com usuários de um pronto atendimento do município de Vitória, ES. Os entrevistados percebem os serviços de urgência e emergência como aqueles nos quais obterão cuidados imediatos com maior facilidade e resolubilidade, já que acessar a APS de uma maneira imediata, rápida e resolutiva se apresentou difícil. A utilização de prontoatendimentos por motivos não urgentes mostrou-se complexa e envolve não apenas a dificuldade de acesso à APS, mas a própria compreensão individual e coletiva do papel de cada equipamento do sistema.

The aim of the present study was to investigate the communication/training strategies and technological tools used by fitness coaches from Brazilian football teams during home-based training according to age categories (professional and youth), competitive level (1st, 2nd and 3rd/4th divisions) and sex (male and female). An online questionnaire was applied to collect data. Eighty-two fitness coaches from sixty-seven male and female football teams (professional and youth teams) answered the questionnaire. Message apps were the main communication route between fitness coaches and players during home-based training. Online spreadsheets were frequently used to control the training. Regarding equipment provided to players for training, 70.7% of male professional players used some equipment. In contrast, most female professional and youth players (both sexes) did not receive any equipment, respectively (p

ABSTRACT After the acute phase of COVID-19, many patients have persistent symptoms or develop sequelae, which leads them to seek physiotherapy rehabilitation services. Therefore, this study aimed to investigate the knowledge and experience of physiotherapists on the assessment and treatment of post-COVID-19 patients. The cross-sectional observational study was carried out with 73 physiotherapists using an online questionnaire about academic background, areas of expertise, knowledge about COVID-19, and knowledge and experience of assessment and treatment resources in post-COVID-19 rehabilitation, in addition to barriers to the care of these patients in the ambulatory care. Most physiotherapists had heard of post-COVID-19 syndrome, however, only 44% felt sufficiently informed about post-COVID-19 rehabilitation. There was a discrepancy between the degree of importance and experience with the frequency of use of assessment resources, especially the use of specific assessment instruments for skeletal muscle strength, mobility, and respiratory function, in addition to scales and questionnaires to assess disabilities, quality of life, and sleep quality. On the other hand, most reported the importance and had sufficient experience to treat post-COVID-19 patients using cheap and accessible resources. However, less than half use techniques related to respiratory muscle training and/or more sophisticated equipment. Thus, we conclude that most physiotherapists recognize the importance and report sufficient experience to assess and treat post-COVID-19 patients, however, there is a discrepancy between the assessment in the biopsychosocial context of the patient and the treatment process.

Enquanto no Norte Global se discute uma crise na Atenção Primária à Saúde, a maioria dos países nunca chegou a constituir sistemas de saúde baseados propriamente numa atenção primária robusta. Nesse cenário, o Brasil apresenta uma tendência mais favorável, com conquistas importantes para a atenção primária e a medicina de família e comunidade nos últimos dez anos. Restam desafios a serem superados para que o Sistema Único de Saúde alcance níveis satisfatórios de acesso a seus serviços, com profissionais adequadamente formados e valorizados pela população.

As políticas públicas de atendimento ao estudante surdo no Brasil reconhecem que o profissional responsável por atuar na mediação linguística é o tradutor e intérprete de Língua Brasileira de Sinais - Libras. No contexto educacional, esse profissional é contratado a partir de diferentes pré-requisitos. Desta forma, este estudo tem como objetivo refletir acerca da formação dos intérpretes educacionais e a sua contratação no ambiente educacional. Como metodologia utilizou-se uma pesquisa documental realizada com dois tipos de fontes: a) ementas dos cursos de graduação em Tradução e Interpretação com Habilitação em Libras; b) editais estaduais de contratação para intérprete educacional. Durante o estudo percebeu-se que o cenário nacional é bastante diverso, manifestando as necessidades, potencialidades e limites de cada contexto regional. Assim, os resultados apontam para a necessidade de discussões sobre a formação para a atuação do intérprete educacional, bem como na falta de consenso nas exigências de contratação deste profissional. Este resultado corrobora com a baixa oferta de cursos superiores de tradução e interpretação na maioria das regiões, o que faz com que as orientações propostas pelos documentos oficiais fiquem distantes da prática. Neste sentido, de maneira emergencial os editais permitem e abrem precedentes para a contratação de profissionais com formação diversa, algumas vezes sem o pré-requisito da formação em nível superior. Esse recurso aplicado em situações emergenciais visa dar conta da educação bilíngue inclusiva e “de qualidade” para os estudantes surdos, solução essa que pode apresentar limites didáticos e metodológicos no exercício da profissão.

Abstract Roadkill is one of the most direct impacts on fauna, leading to numerous direct and indirect impacts. Road-killed animals could serve as a resource for research and teaching purposes, but first, they need to have their tissues preserved by some technique. Plastination is one of the most modern techniques for the conservation of biological tissues, its principle is the replacement of body fluids by a curable polymer, resulting in a non-toxic, odorless, and manipulable material. So the main aim of this work was to compose a collection of plastinated roadkill victims from the Biological Reserve Sooretama region, adapting the standard protocol to the different taxa worked here (Avialae, Reptilia, Amphibia, and Mammalia). For this, 42 animals were used, previously frozen and stored in the Sooretama Biological Reserve and Vale Natural Reserve until they were sent for processing. Upon arriving at the Plastination laboratory at the Federal University of Espírito Santo, the animals underwent the 4 stages of plastination: fixation, dehydration, forced impregnation, and chemical cure; some adaptations were made to improve the final specimens. The results proved that the plastination of these animals is extremely viable in generating satisfactory results. Given the results obtained, it becomes clear how plastination can become an alternative to implement in the composition of biological collections using roadkill animals. The animals prepared in this work comprise an unprecedented collection available for different applications, such as exhibitions, practical classes, and research.