1. TIRR regulates mRNA export and association with P-bodies in response to DNA damage.
- Author
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Glossop MS, Chelysheva I, Ketley RF, Alagia A, and Gullerova M
- Subjects
- Humans, Karyopherins metabolism, Karyopherins genetics, Exportin 1 Protein, Active Transport, Cell Nucleus, Receptors, Cytoplasmic and Nuclear metabolism, Receptors, Cytoplasmic and Nuclear genetics, DNA Breaks, Double-Stranded, Cell Nucleus metabolism, Cell Nucleus genetics, DNA Repair, Nuclear Export Signals genetics, RNA Processing, Post-Transcriptional, DEAD-box RNA Helicases, RNA, Messenger metabolism, RNA, Messenger genetics, DNA Damage, RNA Transport, RNA-Binding Proteins metabolism, RNA-Binding Proteins genetics
- Abstract
To ensure the integrity of our genetic code, a coordinated network of signalling and repair proteins, known as the DNA damage response (DDR), detects and repairs DNA insults, the most toxic being double-strand breaks (DSBs). Tudor interacting repair regulator (TIRR) is a key factor in DSB repair, acting through its interaction with p53 binding protein 1 (53BP1). TIRR is also an RNA binding protein, yet its role in RNA regulation during the DDR remains elusive. Here, we show that TIRR selectively binds to a subset of messenger RNAs (mRNAs) in response to DNA damage. Upon DNA damage, TIRR interacts with the nuclear export protein Exportin-1 through a nuclear export signal. Furthermore, TIRR plays a crucial role in the modulation of RNA processing bodies (PBs). TIRR itself and TIRR-bound RNA co-localize with PBs, and TIRR depletion results in nuclear RNA retention and impaired PB formation. We also suggest a potential link between TIRR-regulated RNA export and efficient DDR. This work reveals intricate involvement of TIRR in orchestrating mRNA nuclear export and storage within PBs, emphasizing its significance in the regulation of RNA-mediated DDR., (© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Published
- 2024
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