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This study discusses the effectiveness of T-beams reinforced with steel fibers and 600 MPa longitudinal reinforcements on negative flexural responses based on experimental and finite element analysis (FEA) methods. Four T-beams reinforced with 400 MPa or 600 MPa reinforcements were manufactured and inversely loaded. Two of the beams were fabricated with 0.75% (by volume) steel fibers as per JGJ/T465-2019, while the rest were cast following the same design but without steel fibers for comparison. The failure modes, the cracking moment and width, and the stress of the longitudinal reinforcements and concrete were compared between the four tested T-beams. In particular, deflection–load curves indicated the improved flexural ability of the specimens with steel fibers and high-yield steel bars. Models of T-beams with longitudinal reinforcements measuring 6, 8, and 12 mm in diameter were simulated and compared with the results of validated models. The FEA results further demonstrate the better flexural performance of T-beams reinforced with steel fibers and longitudinal reinforcements. Both materials can increase the overall performance of inverted steel fiber-reinforced concrete T-beams, including cracking load, crack width, ultimate load, and deflection. However, the findings showed that different diameters of longitudinal reinforcements generated different levels of effectiveness.