1. scMicrobe PTA: near complete genomes from single bacterial cells
- Author
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Bowers, Robert M, Gonzalez-Pena, Veronica, Wardhani, Kartika, Goudeau, Danielle, Blow, Matthew James, Udwary, Daniel, Klein, David, Vill, Albert C, Brito, Ilana L, Woyke, Tanja, Malmstrom, Rex R, and Gawad, Charles
- Subjects
Microbiology ,Biological Sciences ,Biotechnology ,Human Genome ,Genetics ,Infection ,single cell genomics ,unculturable bacteria ,microbiome ,microbial ecology ,primary template-directed amplification ,multiple displacement amplification ,whole genome amplification ,microbiome sequencing ,mobile genetic elements ,biosynthetic gene clusters ,Ecology - Abstract
Microbial genomes produced by standard single-cell amplification methods are largely incomplete. Here, we show that primary template-directed amplification (PTA), a novel single-cell amplification technique, generated nearly complete genomes from three bacterial isolate species. Furthermore, taxonomically diverse genomes recovered from aquatic and soil microbiomes using PTA had a median completeness of 81%, whereas genomes from standard multiple displacement amplification-based approaches were usually
- Published
- 2024