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Black spot caused by Alternaria alternata is one of the most common postharvest diseases in fruit and vegetables. A comprehensive investigation into its pathogenicity mechanism is imperative in order to propose a targeted and effective control strategy. The effect of nitric oxide (NO) on the pathogenicity of A. alternata and its underlying mechanism was studied. The results showed that treatment with 0.5 mM L−1 of sodium nitroprusside (SNP) (NO donor) increased the lesion diameter of A. alternata in vivo and in vitro, which was 22.8% and 13.2% higher than that of the control, respectively. Exogenous NO treatment also induced endogenous NO accumulation by activating nitric oxide synthase (NOS). In addition, NO triggered an increase in reactive oxygen species (ROS) levels. NO enhanced activities and gene expression levels of NADPH oxidase (NOX), superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione peroxidase (GPX), and glutathione reductase (GR). Moreover, NO stimulated cell wall degrading enzymes by activating the corresponding gene expression in vivo and in vitro. These results suggested that exogenous NO promoted the pathogenicity of A. alternata by inducing ROS accumulation and activating antioxidants and cell wall degrading enzymes. The present results could establish a theoretical foundation for the targeted control of the black spot disease in pear fruit. [ABSTRACT FROM AUTHOR]

As crucial water conservancy projects, ship locks play a key role in flood control, shipping, water resource allocation, and promoting regional economic development, making them an indispensable part of the modern water transportation system. Utilizing satellite remote sensing for lock extraction can significantly reduce manual workload and costs, assist in the daily dynamic maintenance of lock hubs, and provide more comprehensive data support for the construction and management of water transport infrastructure. In this context, this paper proposes a new method for ship lock object extraction. Leveraging fuzzy theory and prior knowledge of locks, the extraction of lock objects is achieved from Gaofen-1 (GF-1) high-resolution remote sensing images. The experimental results demonstrate that the proposed algorithm can effectively extract small lock objects in remote sensing images, achieving an average extraction accuracy of 80.9% in the study area. [ABSTRACT FROM AUTHOR]

Sulfoxides have emerged as pivotal constituents in modern carbohydrate chemistry. As anomeric leaving groups, sulfinyl moieties may occupy positions directly at the anomeric position or at a more remote site. This feature article is focused on the evolution and notable advancements of glycosyl sulfoxide donors in glycosylation reactions. Its objective is to elucidate the obstacles and prospects within this evolving research domain, with the aim of enhancing comprehension and progress in the field of carbohydrate chemistry. [ABSTRACT FROM AUTHOR]